PMID- 17784877
OWN - NLM
STAT- MEDLINE
DCOM- 20071221
LR  - 20210904
IS  - 1015-6305 (Print)
IS  - 1750-3639 (Electronic)
IS  - 1015-6305 (Linking)
VI  - 17
IP  - 4
DP  - 2007 Oct
TI  - Presence of HSV-1 immediate early genes and clonally expanded T-cells with a 
      memory effector phenotype in human trigeminal ganglia.
PG  - 389-98
AB  - The latent persistence of herpes simplex virus type 1 (HSV-1) in human trigeminal 
      ganglia (TG) is accompanied by a chronic CD8 T-cell infiltrate. The focus of the 
      current work was to look for HSV-1 transcription activity as a potential trigger 
      of the immune response and to characterize the immune cell infiltrates by this 
      feature. We combined in situ hybridization, laser cutting microscopy, and single 
      cell RT-PCR to demonstrate the expression of the HSV-1 immediate early (IE) genes 
      ICP0 and ICP4 in human trigeminal neurons. Using CDR3 spectratyping, we showed 
      that the infiltrating T-cells are clonally expanded, indicating an antigen-driven 
      immune response. Moreover, the persisting CD8+ T-cells had features of the memory 
      effector phenotype. The voltage-gated potassium channel Kv1.3, a marker of 
      chronic activated memory effector cells, and the chemokines CCL5 and CXCL10 were 
      expressed by a subpopulation of infiltrating cells. The corresponding chemokine 
      receptors CCR5 and CXCR3 were co-expressed on virtually all CD8 T-cells. In 
      addition, T-cells expressed granzymes and perforin. In contrast to animal models 
      of HSV-1 latency, hardly any FoxP3-positive regulatory T-cells were detected in 
      human TG. Thus, HSV-1 IE genes are expressed in human TG and the infiltrating 
      T-cells bear several characteristics that suggest viral antigenic stimulation.
FAU - Derfuss, Tobias
AU  - Derfuss T
AD  - Institute of Clinical Neuroimmunology, University Clinic, Munich, Germany. 
      tsderfus@neuro.mpg.de
FAU - Segerer, Stephan
AU  - Segerer S
FAU - Herberger, Simone
AU  - Herberger S
FAU - Sinicina, Inga
AU  - Sinicina I
FAU - Hufner, Katharina
AU  - Hufner K
FAU - Ebelt, Kathleen
AU  - Ebelt K
FAU - Knaus, Hans-Gunther
AU  - Knaus HG
FAU - Steiner, Israel
AU  - Steiner I
FAU - Meinl, Edgar
AU  - Meinl E
FAU - Dornmair, Klaus
AU  - Dornmair K
FAU - Arbusow, Viktor
AU  - Arbusow V
FAU - Strupp, Michael
AU  - Strupp M
FAU - Brandt, Thomas
AU  - Brandt T
FAU - Theil, Diethilde
AU  - Theil D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070904
PL  - Switzerland
TA  - Brain Pathol
JT  - Brain pathology (Zurich, Switzerland)
JID - 9216781
RN  - 0 (Chemokines)
RN  - 0 (Kv1.3 Potassium Channel)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Chemokines/immunology/metabolism
MH  - Chemotaxis, Leukocyte/genetics/immunology
MH  - Clone Cells/immunology/virology
MH  - Female
MH  - Gene Expression Regulation, Viral/genetics
MH  - Genes, Immediate-Early/*genetics
MH  - Genes, Viral/genetics
MH  - Herpes Simplex/*genetics/physiopathology/*virology
MH  - Herpesvirus 1, Human/*genetics/immunology
MH  - Humans
MH  - Immunologic Memory/genetics/immunology
MH  - Kv1.3 Potassium Channel/metabolism
MH  - Male
MH  - Middle Aged
MH  - Neurons, Afferent/immunology/virology
MH  - Phenotype
MH  - Receptors, Chemokine/immunology/metabolism
MH  - T-Lymphocytes/immunology/*virology
MH  - Trigeminal Ganglion/cytology/immunology/*virology
MH  - Virus Latency/genetics/immunology
PMC - PMC8095593
EDAT- 2007/09/06 09:00
MHDA- 2007/12/22 09:00
PMCR- 2007/09/04
CRDT- 2007/09/06 09:00
PHST- 2007/09/06 09:00 [pubmed]
PHST- 2007/12/22 09:00 [medline]
PHST- 2007/09/06 09:00 [entrez]
PHST- 2007/09/04 00:00 [pmc-release]
AID - BPA088 [pii]
AID - 10.1111/j.1750-3639.2007.00088.x [doi]
PST - ppublish
SO  - Brain Pathol. 2007 Oct;17(4):389-98. doi: 10.1111/j.1750-3639.2007.00088.x. Epub 
      2007 Sep 4.