PMID- 1779884
OWN - NLM
STAT- MEDLINE
DCOM- 19920309
LR  - 20190912
IS  - 0026-2862 (Print)
IS  - 0026-2862 (Linking)
VI  - 42
IP  - 3
DP  - 1991 Nov
TI  - Dynamics of leukocyte-endothelium interactions in the splanchnic 
      microcirculation.
PG  - 288-304
AB  - In vivo dynamics of the interaction between leukocytes and the endothelium 
      following direct activation of the white blood cells (WBCs), apart from possible 
      endothelial cell activation, were studied in arterioles, capillaries, and venules 
      of splanchnic tissue (rabbit omentum). WBCs were isolated using either density 
      gradient or centrifugation techniques, labeled with fluorescent dyes, and exposed 
      to physiological solutions with or without the chemoactivator 
      N-formyl-methionyl-leucyl-phenlyalanine (FMLP). WBCs isolated using standard 
      density gradient separation techniques rapidly disappeared from the circulating 
      pool following a bolus injection and were sequestered in lung microvessels. The 
      centrifugation technique produced cells that circulated for at least 60 min. WBCs 
      directly activated with FMLP adhered to venular endothelium but not to arteriolar 
      endothelium, suggesting that differences in hydrodynamics in the arteriolar and 
      venular network or fundamental differences between arteriolar and venular 
      endothelia may explain the lack of leukocyte-endothelium adhesion (LEA) in 
      arterioles. WBCs pretreated with FMLP had significantly longer attachment times 
      than nontreated cells, 13.4 and 2.5 sec respectively, which may be indicative of 
      specific receptor chemistry. Similarities in the LEA attachment-detachment 
      process for splanchnic tissue with that previously reported for lymphoid tissue 
      suggest that a fundamental process of cell to cell interaction may exist in all 
      tissues.
FAU - House, S D
AU  - House SD
AD  - Department of Biology, College of Arts and Sciences, Seton Hall University, South 
      Orange, New Jersey 07079.
FAU - Lipowsky, H H
AU  - Lipowsky HH
LA  - eng
GR  - HL-28381/HL/NHLBI NIH HHS/United States
GR  - HL-39286/HL/NHLBI NIH HHS/United States
GR  - R29 HL-44914/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Communication/*physiology
MH  - Cell Division/drug effects
MH  - Centrifugation, Density Gradient
MH  - Endothelium, Vascular/drug effects/*physiology
MH  - Female
MH  - Leukocytes/drug effects/*physiology
MH  - Male
MH  - Microcirculation/cytology/physiology
MH  - Omentum/blood supply
MH  - Rabbits
MH  - Splanchnic Circulation/*physiology
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1016/0026-2862(91)90063-h [doi]
PST - ppublish
SO  - Microvasc Res. 1991 Nov;42(3):288-304. doi: 10.1016/0026-2862(91)90063-h.