PMID- 1779975
OWN - NLM
STAT- MEDLINE
DCOM- 19920310
LR  - 20201226
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 5
IP  - 11
DP  - 1991 Nov
TI  - Interleukin-1 alpha and tumor necrosis factor-alpha (TNF alpha) inhibit growth 
      and induce TNF messenger RNA in MCF-7 human breast cancer cells.
PG  - 1740-7
AB  - We studied the effects of interleukin-1 alpha (IL-1) and tumor necrosis 
      factor-alpha (TNF), alone and in combination, on MCF-7 breast cancer cells to 
      determine whether these cytokines alter cell growth, TNF gene expression, and TNF 
      secretion. We found that IL-1 alone and TNF alone inhibited cell growth in a 
      dose-dependent manner. Each cytokine arrested growth in the G0/G1 phase of the 
      cell cycle, with maximum growth inhibition at 1000 U/ml (P less than 0.05) and 
      100 U/ml (P less than 0.01), respectively. However, the combination of these two 
      cytokines did not result in greater growth inhibition or a greater percentage of 
      cells arrested in the G0/G1 phase of the cell cycle compared with each cytokine 
      alone. We examined the effect of exogenous IL-1 and TNF on TNF gene expression by 
      Northern blot analysis. In the absence of any cytokine, these cells do not 
      express TNF mRNA. Exposure to IL-1 (1000 U/ml) induced TNF mRNA at 3 h; however, 
      mRNA levels diminished thereafter to barely detectable levels by 24 h. Exposure 
      to TNF (1000 U/ml) also induced TNF mRNA at 3 h, but in contrast to IL-1, the 
      level of enhanced expression persisted at these levels through 72 h of exposure. 
      Secretion of TNF by these cells is induced by exogenous TNF, but not by IL-1. 
      IL-1 and TNF in combination do not produce greater inhibition of growth, greater 
      amounts of TNF mRNA at 3 h, or greater secretion of TNF than that produced by TNF 
      alone.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Sgagias, M K
AU  - Sgagias MK
AD  - Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892.
FAU - Kasid, A
AU  - Kasid A
FAU - Danforth, D N Jr
AU  - Danforth DN Jr
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
EIN - Mol Endocrinol 1992 Apr;6(4):620
MH  - Blotting, Northern
MH  - Breast Neoplasms
MH  - Cell Cycle/drug effects
MH  - Cell Division/*drug effects
MH  - Cell Line
MH  - DNA, Neoplasm/analysis
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Kinetics
MH  - RNA, Messenger/drug effects/*genetics/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*genetics/*pharmacology
EDAT- 1991/11/11 19:15
MHDA- 2001/03/28 10:01
CRDT- 1991/11/11 19:15
PHST- 1991/11/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1991/11/11 19:15 [entrez]
AID - 10.1210/mend-5-11-1740 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1991 Nov;5(11):1740-7. doi: 10.1210/mend-5-11-1740.