PMID- 17802861
OWN - NLM
STAT- MEDLINE
DCOM- 20071018
LR  - 20190917
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 56
IP  - 3
DP  - 2007
TI  - Diversity of ammonia oxidising bacteria in a vertical flow constructed wetland.
PG  - 241-7
AB  - Vertical flow constructed wetlands (VFCWs) with intermittent loading are very 
      suitable for nitrification. Ammonia oxidising bacteria (AOB) are the limiting 
      step of nitration. Therefore the AOB community of a full-scale VFCW, receiving 
      municipal wastewater, was investigated within this study. The diversity of the 
      functional gene encoding the alpha-subunit of the ammonia monooxygenase (amoA), 
      present only in AOB, was assessed by denaturing gradient gel electrophoresis 
      (DGGE). Only very few amoA sequence types dominated the wetland filter substrate; 
      nevertheless a stable nitrification performance could be observed. During the 
      cold season the nitrification was slightly reduced, but it has been shown that 
      the same AOB could be identified. No spatial AOB pattern could be observed within 
      the filter body of the VFCW. The most prominent bands were excised from DGGE gels 
      and sequenced. Sequence analyses revealed two dominant AOB lineages: Nitrosomonas 
      europaea/"Nitrosococcus mobilis" and Nitrosospira. Species of the Nitrosomonas 
      lineage are commonly found in conventional wastewater treatment plants (WWTPs). 
      In contrast, members of the Nitrosospira lineage are rarely present in WWTPs. Our 
      observations indicate that the AOB community in this VFCW is similar to that 
      found in horizontal flow constructed wetlands, but differs from common WWTPs 
      regarding the presence of Nitrosospira.
FAU - Tietz, A
AU  - Tietz A
AD  - Institute of Sanitary Engineering and Water Pollution Control, BOKU--University 
      of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A-1190 
      Vienna, Austria. alexandra.tietz@boku.ac.at
FAU - Hornek, R
AU  - Hornek R
FAU - Langergraber, G
AU  - Langergraber G
FAU - Kreuzinger, N
AU  - Kreuzinger N
FAU - Haberl, R
AU  - Haberl R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Genetic Variation
MH  - Nitrogen/isolation & purification
MH  - Nitrosomonadaceae/*enzymology/isolation & purification/metabolism
MH  - Nitrosomonas/enzymology/isolation & purification/metabolism
MH  - Oxidoreductases/*genetics
MH  - Waste Disposal, Fluid
MH  - Water Microbiology
MH  - Water Movements
MH  - Water Purification/*methods
MH  - *Wetlands
EDAT- 2007/09/07 09:00
MHDA- 2007/10/19 09:00
CRDT- 2007/09/07 09:00
PHST- 2007/09/07 09:00 [pubmed]
PHST- 2007/10/19 09:00 [medline]
PHST- 2007/09/07 09:00 [entrez]
AID - 10.2166/wst.2007.505 [doi]
PST - ppublish
SO  - Water Sci Technol. 2007;56(3):241-7. doi: 10.2166/wst.2007.505.