PMID- 17803785
OWN - NLM
STAT- MEDLINE
DCOM- 20080103
LR  - 20091119
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 9
IP  - 10
DP  - 2007 Oct
TI  - Candidatus Symbiothrix dinenymphae: bristle-like Bacteroidales ectosymbionts of 
      termite gut protists.
PG  - 2631-5
AB  - Many reports have stated that flagellated protists in termite guts harbour 
      ectosymbiotic spirochetes on their cell surface. In this study, we describe 
      another bristle-like ectosymbiont affiliated with the order Bacteroidales. The 
      16S rRNA phylotype Rs-N74 predominates among Bacteroidales clones obtained from 
      the gut of the termite Reticulitermes speratus. An Rs-N74 phylotype-specific 
      probe was designed in this study and used for detection of the corresponding 
      bacteria in the gut by fluorescence in situ hybridization (FISH) analysis. 
      Surprisingly, the signals were detected specifically from the bristle-like 
      'appendages' of various flagellate species belonging to the genus Dinenympha; 
      these 'appendages' had been believed to be spirochetal ectosymbionts or 
      structures of the protists. The Rs-N74 bacteria attached to the cell surface of 
      the protists by a tip and coexisted with the spirochetal ectosymbionts. An 
      electron micrograph revealed their morphology to be similar to a typical 
      Bacteroidales bacterium. This bacterium is proposed to represent a novel genus 
      and species, 'Candidatus Symbiothrix dinenymphae', phylogenetically affiliated 
      with a cluster consisting exclusively of uncultured strains from termite guts. A 
      Bacteroidales-specific probe for FISH further revealed that this type of 
      symbiosis exists also in various other protists, including parabasalids and 
      oxymonads, and is widespread in termite guts.
FAU - Hongoh, Yuichi
AU  - Hongoh Y
AD  - Environmental Molecular Biology Laboratory, RIKEN, Saitama 351-0198, Japan. 
      yhongo@riken.jp
FAU - Sato, Tomoyuki
AU  - Sato T
FAU - Noda, Satoko
AU  - Noda S
FAU - Ui, Sadaharu
AU  - Ui S
FAU - Kudo, Toshiaki
AU  - Kudo T
FAU - Ohkuma, Moriya
AU  - Ohkuma M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteroidetes/*growth & development/isolation & purification
MH  - Eukaryota/*microbiology
MH  - Gastrointestinal Tract/microbiology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Isoptera/metabolism/*microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Symbiosis
EDAT- 2007/09/07 09:00
MHDA- 2008/01/04 09:00
CRDT- 2007/09/07 09:00
PHST- 2007/09/07 09:00 [pubmed]
PHST- 2008/01/04 09:00 [medline]
PHST- 2007/09/07 09:00 [entrez]
AID - EMI1365 [pii]
AID - 10.1111/j.1462-2920.2007.01365.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2007 Oct;9(10):2631-5. doi: 10.1111/j.1462-2920.2007.01365.x.