PMID- 17804251
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20071010
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 39
IP  - 2
DP  - 2007 Aug
TI  - Identification of peptide inhibitors of transforming growth factor beta 1 using a 
      phage-displayed peptide library.
PG  - 106-15
AB  - Pathologies such as liver fibrosis and scleroderma are characterized by harmful 
      levels of transforming growth factor beta 1 (TGFbeta1). These levels could be 
      neutralized if inhibitors of this cytokine were available. With this aim we 
      searched for peptides with binding affinity for TGFbeta1 using a phage-displayed 
      random 15-mer peptide library. Some peptides thus identified blocked activity of 
      TGFbeta1 in vitro, as measured by their capacity to restore growth of Mv-1-Lu 
      cells in presence of added TGFbeta1. Also, they inhibited TGFbeta1-dependent 
      expression of collagen type I mRNA in liver of mice orally insulted with CCl(4). 
      Intraperitoneal administration of 50 microg of peptide P17 (the most active 
      15-mer peptide, also referred to as P17(1-15)) inhibited expression of collagen 
      type I mRNA by almost 100%. Interestingly, titration experiments showed that 
      P17(1-12) (a peptide encompassing the first 12 amino acids of P17) was 
      approximately four times more active than P17. These results suggest that both 
      peptides, as well as others reported here, may be of therapeutic interest in 
      processes requiring control of undesired high levels of TGFbeta1.
FAU - Dotor, Javier
AU  - Dotor J
AD  - Division of Hepatology and Gene Therapy, Center for Applied Medical Research 
      (CIMA), University of Navarra, Avda. Pio XII, 55-31008-Pamplona, Spain. 
      jdotor@unav.es
FAU - Lopez-Vazquez, Ana B
AU  - Lopez-Vazquez AB
FAU - Lasarte, Juan J
AU  - Lasarte JJ
FAU - Sarobe, Pablo
AU  - Sarobe P
FAU - Garcia-Granero, Marta
AU  - Garcia-Granero M
FAU - Riezu-Boj, Jose I
AU  - Riezu-Boj JI
FAU - Martinez, Alfonso
AU  - Martinez A
FAU - Feijoo, Esperanza
AU  - Feijoo E
FAU - Lopez-Sagaseta, Jacinto
AU  - Lopez-Sagaseta J
FAU - Hermida, Jose
AU  - Hermida J
FAU - Prieto, Jesus
AU  - Prieto J
FAU - Borras-Cuesta, Francisco
AU  - Borras-Cuesta F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070904
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Liver/injuries/pathology
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Peptide Library
MH  - Peptides/*pharmacology
MH  - Protein Binding
MH  - Rats
MH  - Rats, Wistar
MH  - Sequence Analysis, DNA
MH  - Sequence Analysis, Protein
MH  - Surface Plasmon Resonance
MH  - Transforming Growth Factor beta1/*antagonists & inhibitors
EDAT- 2007/09/07 09:00
MHDA- 2008/01/19 09:00
CRDT- 2007/09/07 09:00
PHST- 2006/11/28 00:00 [received]
PHST- 2007/06/08 00:00 [revised]
PHST- 2007/06/18 00:00 [accepted]
PHST- 2007/09/07 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/09/07 09:00 [entrez]
AID - S1043-4666(07)00161-5 [pii]
AID - 10.1016/j.cyto.2007.06.004 [doi]
PST - ppublish
SO  - Cytokine. 2007 Aug;39(2):106-15. doi: 10.1016/j.cyto.2007.06.004. Epub 2007 Sep 
      4.