PMID- 17823239 OWN - NLM STAT- MEDLINE DCOM- 20080326 LR - 20071217 IS - 1549-4918 (Electronic) IS - 1066-5099 (Linking) VI - 25 IP - 12 DP - 2007 Dec TI - Neural cell adhesion molecule polysialylation enhances the sensitivity of embryonic stem cell-derived neural precursors to migration guidance cues. PG - 3016-25 AB - The development of stem cell-based neural repair strategies requires detailed knowledge on the interaction of migrating donor cells with the host brain environment. Here we report that overexpression of polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), in embryonic stem (ES) cell-derived glial precursors (ESGPs) strikingly modifies their migration behavior in response to guidance cues. ESGPs transduced with a retrovirus encoding the polysialyltransferase STX exhibit enhanced migration in monolayer cultures and an increased penetration of organotypic slice cultures. Chemotaxis assays show that overexpression of PSA results in an enhanced chemotactic migration toward gradients of a variety of chemoattractants, including fibroblast growth factor 2 (FGF2), platelet-derived growth factor, and brain-derived neurotrophic factor (BDNF), and that this effect is mediated via the phosphatidylinositol 3'-kinase (PI3K) pathway. Moreover, PSA-overexpressing ESGPs also exhibit an enhanced chemotactic response to tissue explants derived from different brain regions. The effect of polysialylation on directional migration is preserved in vivo. Upon transplantation into the adult striatum, PSA-overexpressing but not control cells display a targeted migration toward the subventricular zone. On the basis of these data, we propose that PSA plays a crucial role in modulating the ability of migrating precursor cells to respond to regional guidance cues within the brain tissue. Disclosure of potential conflicts of interest is found at the end of this article. FAU - Glaser, Tamara AU - Glaser T AD - Institute of Reconstructive Neurobiology, University of Bonn Life and Brain Center, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany. FAU - Brose, Claudia AU - Brose C FAU - Franceschini, Isabelle AU - Franceschini I FAU - Hamann, Katja AU - Hamann K FAU - Smorodchenko, Alina AU - Smorodchenko A FAU - Zipp, Frauke AU - Zipp F FAU - Dubois-Dalcq, Monique AU - Dubois-Dalcq M FAU - Brustle, Oliver AU - Brustle O LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070906 PL - England TA - Stem Cells JT - Stem cells (Dayton, Ohio) JID - 9304532 RN - 0 (Neural Cell Adhesion Molecules) RN - 0 (Sialic Acids) RN - 0 (polysialic acid) SB - IM MH - Animals MH - Cells, Cultured MH - Chemotaxis/*physiology MH - *Cues MH - Embryonic Stem Cells/chemistry/*cytology/*metabolism MH - Mice MH - Neural Cell Adhesion Molecules/*metabolism/physiology MH - Neurons/chemistry/*cytology/*metabolism MH - Rats MH - Sialic Acids/*metabolism/physiology EDAT- 2007/09/08 09:00 MHDA- 2008/03/28 09:00 CRDT- 2007/09/08 09:00 PHST- 2007/09/08 09:00 [pubmed] PHST- 2008/03/28 09:00 [medline] PHST- 2007/09/08 09:00 [entrez] AID - 2007-0218 [pii] AID - 10.1634/stemcells.2007-0218 [doi] PST - ppublish SO - Stem Cells. 2007 Dec;25(12):3016-25. doi: 10.1634/stemcells.2007-0218. Epub 2007 Sep 6.