PMID- 17823392
OWN - NLM
STAT- MEDLINE
DCOM- 20071129
LR  - 20191210
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 19
IP  - 5
DP  - 2007 Sep
TI  - Evaluation of surveillance protocols for detecting porcine reproductive and 
      respiratory syndrome virus infection in boar studs by simulation modeling.
PG  - 492-501
AB  - Because porcine reproductive and respiratory syndrome virus (PRRSV) can be 
      transmitted through semen, PRRSV-free boar studs need to be routinely monitored 
      to rapidly detect any potential PRRSV introduction. However, current protocols 
      for monitoring PRRSV in boar studs are diverse, sometimes very costly, and their 
      effectiveness has not been quantified. The objective of this study was to 
      evaluate the ability of different monitoring protocols to detect PRRSV 
      introduction into a negative boar stud by using a simulation modeling approach. A 
      stochastic transmission model was constructed to simulate the spread of PRRSV in 
      a typical negative boar stud in the USA (herd size of 200 boars, 60% annual 
      replacement) and the performance of monitoring protocols by using different 
      sample sizes (10, 30, and 60 samples), sampling frequency (3 times a week, 
      weekly, and biweekly), and diagnostic procedures (PCR on semen, PCR on serum, 
      ELISA on serum, and both PCR and ELISA on serum). The monitoring protocols were 
      evaluated in terms of the time from PRRSV introduction into the boar stud to 
      PRRSV detection. Protocols that used PCR on serum detected the PRRSV introduction 
      earlier than protocols that used PCR on semen, and these were earlier than those 
      that used ELISA on serum. The most intensive protocol evaluated (testing 60 boars 
      3 times a week by PCR on serum) would need 13 days to detect 95% of the PRRSV 
      introductions. These results support field observations, suggesting that an 
      intensive monitoring protocol needs to be in place in a boar stud to quickly 
      detect a PRRSV introduction.
FAU - Rovira, Albert
AU  - Rovira A
AD  - Department of Veterinary Population Medicine, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, MN 55108, USA. rove0010@umn.edu
FAU - Reicks, Darwin
AU  - Reicks D
FAU - Munoz-Zanzi, Claudia
AU  - Munoz-Zanzi C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Animals
MH  - *Computer Simulation
MH  - Male
MH  - *Models, Biological
MH  - Models, Statistical
MH  - Population Surveillance
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology
MH  - Porcine respiratory and reproductive syndrome virus/isolation & purification
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2007/09/08 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/09/08 09:00
PHST- 2007/09/08 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/09/08 09:00 [entrez]
AID - 19/5/492 [pii]
AID - 10.1177/104063870701900506 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2007 Sep;19(5):492-501. doi: 10.1177/104063870701900506.