PMID- 17823393
OWN - NLM
STAT- MEDLINE
DCOM- 20071129
LR  - 20220408
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 19
IP  - 5
DP  - 2007 Sep
TI  - Evaluation of the sensitivity of reverse-transcription polymerase chain reaction 
      to detect porcine reproductive and respiratory syndrome virus on individual and 
      pooled samples from boars.
PG  - 502-9
AB  - Boar studs are continuously monitored for the presence of porcine reproductive 
      and respiratory syndrome virus (PRRSV) by testing different biological samples by 
      reverse-transcription polymerase chain reaction (RT-PCR). In most cases, samples 
      are run in pools, even though the impact of pooling on the sensitivity of RT-PCR 
      is unknown. The objective of this study was to evaluate the feasibility of using 
      PCR on pooled samples through the estimation of the sensitivity of RT-PCR on 
      different biological samples run individually, in pools of 3 and in pools of 5. 
      Twenty-nine boars were inoculated with a low virulent PRRSV isolate. Serum, blood 
      swab, and semen samples were obtained from each boar every 2 to 3 days for 2 
      weeks. Each sample was tested by RT-PCR undiluted or diluted 1:3 and 1:5 with 
      negative samples. Eleven of the 29 boars did not appear to get infected from the 
      inoculum, as evidenced by no seroconversion 15 days after inoculation. Data from 
      the other 18 boars showed that serum was the best sample to detect PRRSV during 
      acute infection, with the blood swab sample performing almost as well. Semen 
      samples failed to detect PRRSV infection in most of the cases. Pooling samples at 
      pool sizes of 3 and 5 resulted in a decrease in the sensitivity of RT-PCR. 
      Sensitivity was reduced by 6% and 8%, respectively, when serum or blood swab 
      samples were run in pools of 5. The impact of pooling on the sensitivity of PCR 
      was higher in samples taken during the beginning of the viremic period.
FAU - Rovira, Albert
AU  - Rovira A
AD  - Department of Veterinary Population Medicine, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, MN 55108, USA. rove0010@umn.edu
FAU - Clement, Travis
AU  - Clement T
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Thompson, Bob
AU  - Thompson B
FAU - Engle, Mark
AU  - Engle M
FAU - Reicks, Darwin
AU  - Reicks D
FAU - Munoz-Zanzi, Claudia
AU  - Munoz-Zanzi C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Animals
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/*blood/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2007/09/08 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/09/08 09:00
PHST- 2007/09/08 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/09/08 09:00 [entrez]
AID - 19/5/502 [pii]
AID - 10.1177/104063870701900507 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2007 Sep;19(5):502-9. doi: 10.1177/104063870701900507.