PMID- 17845323
OWN - NLM
STAT- MEDLINE
DCOM- 20071211
LR  - 20171116
IS  - 1341-8076 (Print)
IS  - 1341-8076 (Linking)
VI  - 33
IP  - 5
DP  - 2007 Oct
TI  - Plasma levels of alpha-defensins 1-3 are an indicator of neutrophil activation in 
      pregnant and post-partum women.
PG  - 645-50
AB  - AIM: In severe preeclampsia and septic shock, excessively activated neutrophils 
      are thought to injure tissue irreversibly. On the other hand, mild neutrophil 
      activation is known to occur during normal pregnancy. The objective of this study 
      was to determine whether elevated plasma levels of alpha-defensins 1-3 could be 
      used as an indicator of neutrophil activation in pregnant and post-partum women. 
      METHODS: Defensin concentrations in 21 non-pregnant women and men, 184 normal 
      pregnant women, and 55 post-partum women were quantified using an enzyme-linked 
      immunosorbent assay (ELISA). The expression of the surface markers, CD11b and 
      Toll-like receptor-4 (TLR-4), on the neutrophils were analyzed by flow cytometry 
      in a cohort of subjects different from that used for the analysis of 
      alpha-defensin levels. RESULTS: The concentrations of alpha-defensins were 
      significantly higher in women that were in labor than in any of the other 
      subjects. These levels diminished after delivery, but remained significantly 
      elevated at one month post-partum. The expression of both CD11b and TLR-4 was 
      significantly higher in women in labor compared to non-pregnant donors 
      (controls). CD11b expression remained high on the third post-partum day, while 
      TLR-4 expression fell to non-pregnant levels. CONCLUSION: Our results suggest 
      that there is a positive association between defensin levels and neutrophil 
      activation in pregnant and post-partum women.
FAU - Okazaki, Takayuki
AU  - Okazaki T
AD  - Department of Obstetrics and Gynecology, Dokkyo Medical University School of 
      Medicine, Tochigi, Japan. okazaki@dokkyomed.ac.jp
FAU - Ota, Yoriko
AU  - Ota Y
FAU - Yuki, Nobuhiro
AU  - Yuki N
FAU - Hayashida, Ayako
AU  - Hayashida A
FAU - Shoda, Akiko
AU  - Shoda A
FAU - Nishikawa, Masayoshi
AU  - Nishikawa M
FAU - Oshima, Kyoko
AU  - Oshima K
FAU - Fukasawa, Ichio
AU  - Fukasawa I
FAU - Watanabe, Hiroshi
AU  - Watanabe H
FAU - Inaba, Noriyuki
AU  - Inaba N
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - J Obstet Gynaecol Res
JT  - The journal of obstetrics and gynaecology research
JID - 9612761
RN  - 0 (CD11b Antigen)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (alpha-Defensins)
SB  - IM
MH  - Adult
MH  - CD11b Antigen/immunology
MH  - Cell Count
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Neutrophil Activation/*immunology
MH  - Neutrophils/*immunology
MH  - Postpartum Period/*blood/immunology
MH  - Pregnancy/*blood/immunology
MH  - Pregnancy Complications/*blood/diagnosis/immunology
MH  - Toll-Like Receptor 4/immunology
MH  - alpha-Defensins/*blood/immunology
EDAT- 2007/09/12 09:00
MHDA- 2007/12/12 09:00
CRDT- 2007/09/12 09:00
PHST- 2007/09/12 09:00 [pubmed]
PHST- 2007/12/12 09:00 [medline]
PHST- 2007/09/12 09:00 [entrez]
AID - JOG626 [pii]
AID - 10.1111/j.1447-0756.2007.00626.x [doi]
PST - ppublish
SO  - J Obstet Gynaecol Res. 2007 Oct;33(5):645-50. doi: 
      10.1111/j.1447-0756.2007.00626.x.