PMID- 17845756
OWN - NLM
STAT- MEDLINE
DCOM- 20100727
LR  - 20221207
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 36
IP  - 7
DP  - 2007 Jul
TI  - [Protein overexpression and gene copy number of EGFR and HER2 in colorectal 
      carcinoma].
PG  - 447-52
AB  - OBJECTIVE: To investigate the protein expression and gene copy number of EGFR and 
      HER2, and the correlation between the two markers in colorectal carcinomas in 
      Chinese. METHOD: Total 42 samples of paraffin-embedded colorectal carcinomas in 
      tissue microarray format were studied by immunohistochemistry (IHC) and 
      fluorescence in situ hybridization (FISH) for EGFR and HER2 protein expression 
      and gene copy number status, respectively. RESULTS: Among 42 cases evaluated, 
      EGFR scores were 0 in 18 cases, 1+ in 10 cases, 2+ in 5 cases and 3+ in 9 cases. 
      HER2 expression was negative in 39 tumors, 1+ in 1 tumor, 2+ in 1 tumor and 3+ in 
      1 tumor. For FISH assessing EGFR, 18 (42.9%) cases showed no apparent copy number 
      changes, including 14 (33.3%) cases of disomy and 4 (9.5%) cases of low trisomy, 
      24 (57.1%) cases showed increased gene copy numbers including high trisomy in 
      3/42 (7.1%), low polysomy in 9/42 (21.4%) and high polysomy in 12/42 (28.6%) 
      cases. Gene amplification of EGFR is not detected. Four of 42 patients (9.5%) had 
      increased HER2 gene copy number, including 3 patients with high polysomy and 1 
      patient with gene amplification. Significant association was not seen between 
      EGFR protein expression and the gene copy number, nor between two markers and 
      tumor differentiation. There was a highly significant concordance between the 
      gene amplification and IHC 3+ for HER2 similar to that of breast cancer. 
      CONCLUSIONS: Protein expression and/or increased gene copy number of EGFR is 
      common in colorectal carcinomas but unrelated to pathological features in this 
      cohort. HER2 protein overexpression and/or gene amplification are rare.
FAU - Zeng, Xuan
AU  - Zeng X
AD  - Department of Pathology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences, Peking Union Medical College, Beijing 100730, China.
FAU - Wang, Peng
AU  - Wang P
FAU - Wu, Sha-fei
AU  - Wu SF
FAU - Gao, Jie
AU  - Gao J
FAU - Liang, Zhi-yong
AU  - Liang ZY
FAU - Liu, Tong-hua
AU  - Liu TH
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Asian People
MH  - Colorectal Neoplasms/genetics/*metabolism/pathology
MH  - ErbB Receptors/genetics/*metabolism
MH  - Female
MH  - *Gene Dosage
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/genetics/*metabolism
EDAT- 2007/09/12 09:00
MHDA- 2010/07/28 06:00
CRDT- 2007/09/12 09:00
PHST- 2007/09/12 09:00 [pubmed]
PHST- 2010/07/28 06:00 [medline]
PHST- 2007/09/12 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2007 Jul;36(7):447-52.