PMID- 17846504
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 24
IP  - 1
DP  - 2007 Aug 31
TI  - Genome-wide examination of chromosomal aberrations in neuroblastoma SH-SY5Y cells 
      by array-based comparative genomic hybridization.
PG  - 105-12
AB  - Most neuroblastoma cells have chromosomal aberrations such as gains, losses, 
      amplifications and deletions of DNA. Conventional approaches like fluorescence in 
      situ hybridization (FISH) or metaphase comparative genomic hybridization (CGH) 
      can detect chromosomal aberrations, but their resolution is low. In this study we 
      used array-based comparative genomic hybridization to identify the chromosomal 
      aberrations in human neuroblastoma SH-SY5Y cells. The DNA microarray consisting 
      of 4000 bacterial artificial chromosome (BAC) clones was able to detect 
      chromosomal regions with aberrations. The SH-SY5Y cells showed chromosomal gains 
      in 1q12 approximately q44 (Chr1:142188905-246084832), 7 (over the whole 
      chromosome), 2p25.3 approximately p16.3 (Chr2:18179-47899074), and 17q 21.32 
      approximately q25.3 (Chr17:42153031-78607159), while chromosomal losses detected 
      were the distal deletion of 1p36.33 (Chr1:552910-563807), 14q21.1 approximately 
      q21.3 (Chr14:37666271- 47282550), and 22q13.1 approximately q13.2 
      (Chr22:36885764-4190 7123). Except for the gain in 17q21 and the loss in 1p36, 
      the other regions of gain or loss in SH-SY5Y cells were newly identified.
FAU - Do, Jin Hwan
AU  - Do JH
AD  - Bio-Food and Drug Research Center, Konkuk University, Chungju 380-701, Korea.
FAU - Kim, In Su
AU  - Kim IS
FAU - Park, Tae-Kyu
AU  - Park TK
FAU - Choi, Dong-Kug
AU  - Choi DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neuroblastoma/*genetics
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Tumor Cells, Cultured
EDAT- 2007/09/12 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/09/12 09:00
PHST- 2007/09/12 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/09/12 09:00 [entrez]
AID - S1016-8478(23)10761-8 [pii]
PST - ppublish
SO  - Mol Cells. 2007 Aug 31;24(1):105-12.