PMID- 17848138
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20181113
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 409
IP  - 1
DP  - 2008 Jan 1
TI  - Genomic location of the human RNA polymerase II general machinery: evidence for a 
      role of TFIIF and Rpb7 at both early and late stages of transcription.
PG  - 139-47
AB  - The functions ascribed to the mammalian GTFs (general transcription factors) 
      during the various stages of the RNAPII (RNA polymerase II) transcription 
      reaction are based largely on in vitro studies. To gain insight as to the 
      functions of the GTFs in living cells, we have analysed the genomic location of 
      several human GTF and RNAPII subunits carrying a TAP (tandem-affinity 
      purification) tag. ChIP (chromatin immunoprecipitation) experiments using 
      anti-tag beads (TAP-ChIP) allowed the systematic localization of the tagged 
      factors. Enrichment of regions located close to the TIS (transcriptional 
      initiation site) versus further downstream TRs (transcribed regions) of nine 
      human genes, selected for the minimal divergence of their alternative TIS, were 
      analysed by QPCR (quantitative PCR). We show that, in contrast with reports using 
      the yeast system, human TFIIF (transcription factor IIF) associates both with 
      regions proximal to the TIS and with further downstream TRs, indicating an in 
      vivo function in elongation for this GTF. Unexpectedly, we found that the Rpb7 
      subunit of RNAPII, known to be required only for the initiation phase of 
      transcription, remains associated with the polymerase during early elongation. 
      Moreover, ChIP experiments conducted under stress conditions suggest that Rpb7 is 
      involved in the stabilization of transcribing polymerase molecules, from 
      initiation to late elongation stages. Together, our results provide for the first 
      time a general picture of GTF function during the RNAPII transcription reaction 
      in live mammalian cells and show that TFIIF and Rpb7 are involved in both early 
      and late transcriptional stages.
FAU - Cojocaru, Marilena
AU  - Cojocaru M
AD  - Institut de Recherches Cliniques de Montreal, 110 avenue des Pins Ouest, 
      Montreal, QC, Canada H2W 1R7.
FAU - Jeronimo, Celia
AU  - Jeronimo C
FAU - Forget, Diane
AU  - Forget D
FAU - Bouchard, Annie
AU  - Bouchard A
FAU - Bergeron, Dominique
AU  - Bergeron D
FAU - Cote, Pierre
AU  - Cote P
FAU - Poirier, Guy G
AU  - Poirier GG
FAU - Greenblatt, Jack
AU  - Greenblatt J
FAU - Coulombe, Benoit
AU  - Coulombe B
LA  - eng
GR  - 14309-3/Canadian Institutes of Health Research/Canada
GR  - 82851-1/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA Primers)
RN  - 0 (Peptides)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (POLR2G protein, human)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 3.6.4.12 (transcription factor TFIIF)
SB  - IM
MH  - Cell Line
MH  - Chromatin Immunoprecipitation
MH  - DNA/metabolism
MH  - DNA Primers/chemistry
MH  - *Gene Expression Regulation
MH  - *Genomics
MH  - Humans
MH  - Peptides/chemistry
MH  - RNA Polymerase II/biosynthesis/*genetics
MH  - Transcription Factors/metabolism
MH  - Transcription Factors, TFII/*genetics
MH  - *Transcription, Genetic
PMC - PMC4498901
MID - CAMS4842
OID - NLM: CAMS4842
EDAT- 2007/09/13 09:00
MHDA- 2008/01/09 09:00
PMCR- 2015/07/10
CRDT- 2007/09/13 09:00
PHST- 2007/09/13 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/09/13 09:00 [entrez]
PHST- 2015/07/10 00:00 [pmc-release]
AID - BJ20070751 [pii]
AID - 10.1042/BJ20070751 [doi]
PST - ppublish
SO  - Biochem J. 2008 Jan 1;409(1):139-47. doi: 10.1042/BJ20070751.