PMID- 17848164
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 122
IP  - 2
DP  - 2007 Oct
TI  - Human dendritic cells transfected with allergen-DNA stimulate specific 
      immunoglobulin G4 but not specific immunoglobulin E production of autologous B 
      cells from atopic individuals in vitro.
PG  - 239-46
AB  - Atopic/allergic diseases are characterized by T helper 2 (Th2)-dominated immune 
      responses resulting in immunoglobulin E (IgE) production. DNA-based 
      immunotherapies have been shown to shift the immune response towards Th1 in 
      animal models. In further studies we showed that human dendritic cells (DC) 
      transfected with allergen-DNA are able to stimulate autologous CD4(+) T cells 
      from atopic individuals to produce Th1 instead of Th2 cytokines and to activate 
      interferon-gamma (IFN-gamma)-producing CD8(+) T cells. The aim of this study was 
      to analyse whether DC transfected with allergen-DNA are also able to influence 
      immunoglobulin production of B cells from atopic donors. For this purpose, human 
      monocyte-derived DC from grass-pollen allergic donors were transfected with an 
      adenovirus encoding the allergen Phleum pratense 1 and cocultured with B cells, 
      autologous CD4(+) T cells, and CD40 ligand-transfected L-cells. B cells receiving 
      help from CD4(+) T cells stimulated with allergen-transfected dendritic cells 
      produced more allergen-specific IgG4 compared to stimulation with allergen 
      protein pulsed DC or medium, while total IgG4 production was not affected. In 
      contrast, specific IgE production was not enhanced by stimulation with 
      allergen-DNA transfected DC compared to medium and inhibited compared to allergen 
      protein-pulsed DC with similar effects on total IgE production in vitro. 
      Allergen-DNA transfected dendritic cells are able to direct the human allergic 
      immune response from Th2-dominance towards Th1 and Tc1 also resulting in 
      decreased IgE and increased IgG4 production.
FAU - Konig, Bettina
AU  - Konig B
AD  - Department of Dermatology, University of Mainz, Mainz, Germany.
FAU - Petersen, Arnd
AU  - Petersen A
FAU - Bellinghausen, Iris
AU  - Bellinghausen I
FAU - Bottcher, Ingo
AU  - Bottcher I
FAU - Becker, Wolf-Meinhard
AU  - Becker WM
FAU - Knop, Jurgen
AU  - Knop J
FAU - Saloga, Joachim
AU  - Saloga J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Plant Proteins)
RN  - 160227-82-7 (PHLPI protein, Phleum pratense)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/genetics/immunology
MH  - B-Lymphocytes/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/*biosynthesis
MH  - Lymphocyte Activation/immunology
MH  - Plant Proteins/immunology
MH  - Rhinitis/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Th1 Cells/immunology
MH  - Transfection
PMC - PMC2266010
EDAT- 2007/09/13 09:00
MHDA- 2007/11/10 09:00
PMCR- 2008/10/01
CRDT- 2007/09/13 09:00
PHST- 2007/09/13 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/09/13 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - IMM2633 [pii]
AID - 10.1111/j.1365-2567.2007.02633.x [doi]
PST - ppublish
SO  - Immunology. 2007 Oct;122(2):239-46. doi: 10.1111/j.1365-2567.2007.02633.x.