PMID- 17848732
OWN - NLM
STAT- MEDLINE
DCOM- 20071015
LR  - 20211203
IS  - 0163-4984 (Print)
IS  - 0163-4984 (Linking)
VI  - 118
IP  - 1
DP  - 2007 Jul
TI  - Effect of supplemental dietary zinc on the mammalian target of rapamycin (mTOR) 
      signaling pathway in skeletal muscle and liver from post-absorptive mice.
PG  - 65-76
AB  - Zinc (Zn) is an essential trace element that functions in cellular signaling. The 
      mammalian target of rapamycin (mTOR) regulates the initiation of protein 
      synthesis. The objective of this study was to determine whether Zn could 
      stimulate protein phosphorylation in the mTOR pathway in vivo. Mice (C57BL/6J, n 
      = 30) were fed Zn marginal diets (ZM, 5 mg/kg) for 4 weeks, followed by fasting 
      (F) and/or refeeding with ZM or Zn supplemental (300 mg/kg, ZS) diets for 3 or 6 
      h. Plasma insulin was greater (P < 0.05) in refed animals as compared to F 
      animals. Protein phosphorylation was detected using multiplex analysis and 
      Western blotting. Multiplex analysis indicated greater (P < 0.05) p70 S6 kinase 
      (p70S6K) and glycogen synthase kinase 3 (GSK-3 alpha/beta) phosphorylation in 
      livers from 6-h refed ZS animals as compared to F animals. Western blots 
      indicated increased (P < 0.05) Akt (Ser 473) phosphorylation in skeletal muscle 
      from animals refed ZS diets for 3 and 6 h as compared to F animals. The ZS diet 
      affected phosphorylation of GSK-3 (alpha/beta) in liver, as 3-h ZS refed animals 
      had greater (P < 0.01) phosphorylation than F animals. These findings indicate 
      that Zn may contribute to the initiation of protein synthesis as a signaling 
      molecule in vivo.
FAU - McClung, James P
AU  - McClung JP
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine (USARIEM), Natick, MA 01760, USA. James.McClung@na.amedd.army.mil
FAU - Tarr, Tyson N
AU  - Tarr TN
FAU - Barnes, Brian R
AU  - Barnes BR
FAU - Scrimgeour, Angus G
AU  - Scrimgeour AG
FAU - Young, Andrew J
AU  - Young AJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Insulin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - *Dietary Supplements
MH  - Glycogen Synthase Kinase 3/metabolism
MH  - Insulin/metabolism
MH  - Liver/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*metabolism
MH  - Phosphorylation
MH  - Protein Kinases/*metabolism
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - Signal Transduction/*physiology
MH  - TOR Serine-Threonine Kinases
MH  - Zinc/*administration & dosage
EDAT- 2007/09/13 09:00
MHDA- 2007/10/16 09:00
CRDT- 2007/09/13 09:00
PHST- 2006/12/04 00:00 [received]
PHST- 2007/01/03 00:00 [revised]
PHST- 2007/01/08 00:00 [accepted]
PHST- 2007/09/13 09:00 [pubmed]
PHST- 2007/10/16 09:00 [medline]
PHST- 2007/09/13 09:00 [entrez]
AID - BTER:118:1:65 [pii]
AID - 10.1007/s12011-007-0018-8 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2007 Jul;118(1):65-76. doi: 10.1007/s12011-007-0018-8.