PMID- 17851144
OWN - NLM
STAT- MEDLINE
DCOM- 20071219
LR  - 20121115
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 858
IP  - 1-2
DP  - 2007 Oct 15
TI  - Liquid chromatography-tandem mass spectrometric method for determination of 
      mosapride citrate in equine tissues.
PG  - 135-42
AB  - A simple method for determination of mosapride citrate and its metabolite, 
      des-p-fluorobenzyl mosapride (M-1), in equine muscle, liver, kidney, adipose 
      tissue and intestine by liquid chromatography-tandem mass spectrometry has been 
      developed. 
      (+/-)-4-Amino-5-chloro-2-ethoxy-N-[[4-(2-chlorobenzyl)morpholinyl]methyl]benzamide 
      was used as an internal standard. The analytes and internal standard were spiked 
      and extracted from tissues by acetonitrile. The chromatographic separation was 
      performed on a reversed-phase TSK-GEL SUPER ODS column with a mobile phase of 
      acetonitrile-0.05% (v/v) formic acid containing 5 mmol/L nonafluoropentanoic acid 
      (2:3, v/v). The method exhibited a large linear range from 0.0005 to 0.2 
      microg/mL for both mosapride citrate and M-1 (r>0.9976). In the intra-day assay 
      (n=5), the relative standard deviations (RSDs) ranged from 1.1 to 7.8% for 
      mosapride citrate and 1.6 to 7.2% for M-1. In the inter-day assay (n=3), the RSDs 
      ranged from 1.0 to 13% for mosapride citrate and 0.8 to 11% for M-1. The 
      extraction recovery at 1.28 microg/g of mosapride citrate from equine tissues 
      ranged from 97 to 107%. The lower limit of quantification for mosapride citrate 
      was found to be 0.004 microg/g. Stability studies were carried out at different 
      storage conditions. The method reported is reliable, precise, and accurate and it 
      has the capacity to be used for determination of mosapride citrate and its 
      metabolite in tissue samples.
FAU - Aoki, Yoichi
AU  - Aoki Y
AD  - Department of Analytical Chemistry, Research Institute for Animal Science in 
      Biochemistry and Toxicology, Sagamihara, Kanagawa 229-1132, Japan.
FAU - Hakamata, Hideki
AU  - Hakamata H
FAU - Igarashi, Yu
AU  - Igarashi Y
FAU - Uchida, Kazunari
AU  - Uchida K
FAU - Kobayashi, Hisato
AU  - Kobayashi H
FAU - Hirayama, Norio
AU  - Hirayama N
FAU - Kotani, Akira
AU  - Kotani A
FAU - Kusu, Fumiyo
AU  - Kusu F
LA  - eng
PT  - Journal Article
DEP - 20070822
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Benzamides)
RN  - 0 (Morpholines)
RN  - I8MFJ1C0BY (mosapride)
SB  - IM
MH  - Adipose Tissue/chemistry
MH  - Animals
MH  - Benzamides/*analysis/standards
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Horses
MH  - Intestines/chemistry
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Morpholines/*analysis/standards
MH  - Muscles/chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2007/09/14 09:00
MHDA- 2007/12/20 09:00
CRDT- 2007/09/14 09:00
PHST- 2007/02/15 00:00 [received]
PHST- 2007/07/26 00:00 [revised]
PHST- 2007/08/13 00:00 [accepted]
PHST- 2007/09/14 09:00 [pubmed]
PHST- 2007/12/20 09:00 [medline]
PHST- 2007/09/14 09:00 [entrez]
AID - S1570-0232(07)00588-0 [pii]
AID - 10.1016/j.jchromb.2007.08.017 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):135-42. doi: 
      10.1016/j.jchromb.2007.08.017. Epub 2007 Aug 22.