PMID- 17868258
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20071008
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 70
IP  - 5
DP  - 2007 Nov
TI  - Reanalysis of sequence-based HLA-A, -B and -Cw typings: how ambiguous is today's 
      SBT typing tomorrow.
PG  - 383-9
AB  - The permanently increasing number of human leukocyte antigen (HLA)-alleles and 
      the growing list of ambiguities require continuous updating of high-resolution 
      HLA typing results. Two different kinds of ambiguities exist: the first, when two 
      or more allele combinations have identical heterozygous sequences, and the 
      second, when differences are located outside the analyzed region. The number of 
      HLA-A, B and C alleles recognized in 1999 was almost tripled in 2006. Two hundred 
      individuals, sequence-based typing (SBT) typed in the period from 1999 to 2002, 
      were reanalyzed using the 2006 database. A final allele typing result of at least 
      four digits was obtained for HLA-A, -B and -C by heterozygous sequencing of exons 
      2 and 3 and, if necessary, additional exons and/or allele-specific sequencing. 
      Storage of the individual sequences in a specially developed database enabled 
      reanalysis with all present and future HLA releases. In the 5-year period HLA-A, 
      -B and -C typing results became ambiguous in 37%, 46% and 41% of the cases. Most 
      were because of differences outside the analyzed region; ambiguities because of 
      different allele combinations with identical heterozygous sequences were present 
      in 7%, 8% and 13% of the HLA-A, -B and -C typings. These results indicate that 
      within 5 years, approximately half of the HLA SBT typings become ambiguous.
FAU - Voorter, C E M
AU  - Voorter CE
AD  - Tissue Typing Laboratory, University Hospital Maastricht, Maastricht, The 
      Netherlands.
FAU - Mulkers, E
AU  - Mulkers E
FAU - Liebelt, P
AU  - Liebelt P
FAU - Sleyster, E
AU  - Sleyster E
FAU - van den Berg-Loonen, E M
AU  - van den Berg-Loonen EM
LA  - eng
PT  - Journal Article
DEP - 20070916
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - *Alleles
MH  - *Databases, Genetic/trends
MH  - Female
MH  - HLA-A Antigens/*genetics
MH  - HLA-B Antigens/*genetics
MH  - HLA-C Antigens/*genetics
MH  - Heterozygote
MH  - *Histocompatibility Testing/trends
MH  - Humans
MH  - Male
MH  - Retrospective Studies
MH  - Sequence Analysis, DNA/trends
EDAT- 2007/09/18 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/09/18 09:00
PHST- 2007/09/18 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/09/18 09:00 [entrez]
AID - TAN921 [pii]
AID - 10.1111/j.1399-0039.2007.00921.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Nov;70(5):383-9. doi: 10.1111/j.1399-0039.2007.00921.x. 
      Epub 2007 Sep 16.