PMID- 17868882
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20131121
IS  - 1054-8807 (Print)
IS  - 1054-8807 (Linking)
VI  - 16
IP  - 5
DP  - 2007 Sep-Oct
TI  - Folic acid reduces chemokine MCP-1 release and expression in rats with 
      hyperhomocystinemia.
PG  - 305-9
AB  - OBJECTIVE: This study aimed to investigate the effects of folate on the monocyte 
      chemoattractant protein-1 (MCP-1) expression and release in rats with 
      hyperhomocystinemia induced by ingestion of excess methionine. METHODS AND 
      RESULTS: Thirty male Sprague-Dawley rats (200+/-20 g) were randomly divided into 
      three groups (n=10 for each group): control group (Control), high-homocystinemia 
      (Hhcy) group, and folate treatment (FA) group. They were fed with a normal 
      regular diet, enriched by 1.7% methionine plus 1.7% methionine and 0.006% folate 
      for 45 days. Our study showed the following: (a) A high methionine diet for 45 
      days is sufficient to induce hyperhomocystinemia; folate supplementation to the 
      rats fed the high-methionine diet prevented an elevation homocysteine (Hcy) 
      levels in the blood (P<.01). (b) Compared with the Control group, the Hhcy group 
      had elevated plasma levels of MCP-1, and Hcy was significantly correlated with 
      MCP-1 (P<.05). (c) The protein and mRNA expression of MCP-1 in the aorta was 
      higher in rats from the Hhcy group than in rats from the Control group. (d) Most 
      important, after folic acid supplementation, the lowering of Hcy levels was 
      accompanied by a marked reduction of MCP-1 expressed in aortae and released from 
      plasma and peripheral blood mononuclear cells (PBMCs) stimulated by oxidized 
      low-density lipoprotein (P<.05, P<.01). CONCLUSION: Folic acid supplementation 
      not only can blunt the rise in Hcy and reduce MCP-1 released from both plasma and 
      PBMCs of rats with hyperhomocystinemia but also can downgrade MCP-1 expression in 
      the aorta of rats with hyperhomocystinemia.
FAU - Li, Ming
AU  - Li M
AD  - Institute of Cardiovasology, Union Hospital, Tongii Medical College, Huazhong 
      Science and Technology University, Hubei, China. liming518888cn@yahoo.com.cn
FAU - Chen, Jian
AU  - Chen J
FAU - Li, Yu-Shu
AU  - Li YS
FAU - Feng, Yi-Bai
AU  - Feng YB
FAU - Zeng, Qiu-Tang
AU  - Zeng QT
LA  - eng
PT  - Journal Article
DEP - 20070511
PL  - United States
TA  - Cardiovasc Pathol
JT  - Cardiovascular pathology : the official journal of the Society for Cardiovascular 
      Pathology
JID - 9212060
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 12001-76-2 (Vitamin B Complex)
RN  - 935E97BOY8 (Folic Acid)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Aorta/*drug effects/metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chemokine CCL2/blood/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Folic Acid/*pharmacology/therapeutic use
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/chemically induced/metabolism/*prevention & control
MH  - Immunohistochemistry
MH  - Leukocytes, Mononuclear/drug effects/*metabolism
MH  - Lipoproteins, LDL/metabolism
MH  - Male
MH  - Methionine
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Vitamin B Complex/*pharmacology/therapeutic use
EDAT- 2007/09/18 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/09/18 09:00
PHST- 2006/10/31 00:00 [received]
PHST- 2007/02/28 00:00 [revised]
PHST- 2007/03/13 00:00 [accepted]
PHST- 2007/09/18 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/09/18 09:00 [entrez]
AID - S1054-8807(07)00051-8 [pii]
AID - 10.1016/j.carpath.2007.03.005 [doi]
PST - ppublish
SO  - Cardiovasc Pathol. 2007 Sep-Oct;16(5):305-9. doi: 10.1016/j.carpath.2007.03.005. 
      Epub 2007 May 11.