PMID- 17869032 OWN - NLM STAT- MEDLINE DCOM- 20081105 LR - 20081121 IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 127 IP - 1-2 DP - 2008 Feb 5 TI - Genetic diversity of porcine reproductive and respiratory syndrome virus strains circulating in Hungarian swine herds. PG - 128-35 AB - Analysis of 37 ORF5 sequences of Hungarian porcine reproductive and respiratory syndrome virus (PRRSV) strains revealed that most of them (35) belonged to the European genotype, forming distinct subgroups, reflecting the exceptional diversity of Eastern European strains. Twelve vaccine-like strains were also found in non-vaccinated animals. Two strains belonged to the American genotype showing 90-91% nucleotide identity to the "Quebec" Canadian reference strain. The analysis of the putative ectodomains and their N-linked glycosylation sites of the vaccine strain and its variants suggested selective pressure on the first ectodomain, by a consistent amino acid change on epitope B and by loosing a glycosylation site in the otherwise conserved N-46 position. FAU - Balka, Gyula AU - Balka G AD - Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent Istvan University, Istvan u. 2, Budapest, Hungary. balka.gyula@aotk.szie.hu FAU - Hornyak, Akos AU - Hornyak A FAU - Balint, Adam AU - Balint A FAU - Kiss, Istvan AU - Kiss I FAU - Kecskemeti, Sandor AU - Kecskemeti S FAU - Bakonyi, Tamas AU - Bakonyi T FAU - Rusvai, Miklos AU - Rusvai M LA - eng SI - GENBANK/DQ366639 SI - GENBANK/DQ366640 SI - GENBANK/DQ366641 SI - GENBANK/DQ366642 SI - GENBANK/DQ366643 SI - GENBANK/DQ366644 SI - GENBANK/DQ366645 SI - GENBANK/DQ366646 SI - GENBANK/DQ366647 SI - GENBANK/DQ366648 SI - GENBANK/DQ366649 SI - GENBANK/DQ366650 SI - GENBANK/DQ366651 SI - GENBANK/DQ366652 SI - GENBANK/DQ366653 SI - GENBANK/DQ366654 SI - GENBANK/DQ366655 SI - GENBANK/DQ366656 SI - GENBANK/DQ366657 SI - GENBANK/DQ366658 SI - GENBANK/EF406336 SI - GENBANK/EF406337 SI - GENBANK/EF406338 SI - GENBANK/EF406339 SI - GENBANK/EF406340 SI - GENBANK/EF406341 SI - GENBANK/EF406342 SI - GENBANK/EF406343 SI - GENBANK/EF406344 SI - GENBANK/EF406345 SI - GENBANK/EF406346 SI - GENBANK/EF406347 SI - GENBANK/EF406348 SI - GENBANK/EF406349 SI - GENBANK/EF406350 SI - GENBANK/EF406351 SI - GENBANK/EF406352 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070807 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Viral Envelope Proteins) RN - 0 (glycoprotein 5, PRRSV) SB - IM MH - Amino Acid Sequence MH - Animals MH - *Genetic Variation MH - Hungary MH - Molecular Sequence Data MH - Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/*virology MH - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & purification MH - Sequence Alignment MH - Swine MH - Viral Envelope Proteins/chemistry/genetics EDAT- 2007/09/18 09:00 MHDA- 2008/11/06 09:00 CRDT- 2007/09/18 09:00 PHST- 2007/05/23 00:00 [received] PHST- 2007/07/24 00:00 [revised] PHST- 2007/08/02 00:00 [accepted] PHST- 2007/09/18 09:00 [pubmed] PHST- 2008/11/06 09:00 [medline] PHST- 2007/09/18 09:00 [entrez] AID - S0378-1135(07)00388-4 [pii] AID - 10.1016/j.vetmic.2007.08.001 [doi] PST - ppublish SO - Vet Microbiol. 2008 Feb 5;127(1-2):128-35. doi: 10.1016/j.vetmic.2007.08.001. Epub 2007 Aug 7.