PMID- 17869536
OWN - NLM
STAT- MEDLINE
DCOM- 20071220
LR  - 20191210
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 18
IP  - 11
DP  - 2007 Nov
TI  - Effect of the addition of diammonium citrate to alpha-cyano-4-hydroxycinnamic 
      acid (CHCA) matrix for the detection of phosphorylated peptide in phosphorylation 
      reactions using cell and tissue lysates.
PG  - 1925-31
AB  - The ionization of phosphorylated peptides is usually suppressed by 
      non-phosphorylated peptides when alpha-cyano-4-hydroxycinnamic acid (CHCA) is 
      used as a matrix for matrix-assisted laser desorption/ionization-time-of-Flight 
      (MALDI-TOF) mass spectrometry analysis. In the present study, we examined the 
      effect of diammonium citrate addition to the CHCA matrix on the detection of 
      phosphorylated peptides. Substrates for protein kinase C (PKC) and c-Src were 
      synthesized and phosphorylated by reaction with cell and tissue lysate samples. 
      The addition of diammonium citrate to the CHCA matrix increased the sensitivity 
      for distinguishing phosphorylated peptides from background noise. However, the 
      effect depended on substrate concentration.
FAU - Kang, Jeong-Hun
AU  - Kang JH
AD  - CREST, Japan Science and Technology Corporation, Kawaguchi, Japan.
FAU - Toita, Riki
AU  - Toita R
FAU - Oishi, Jun
AU  - Oishi J
FAU - Niidome, Takuro
AU  - Niidome T
FAU - Katayama, Yoshiki
AU  - Katayama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070815
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Coumaric Acids)
RN  - 0 (Peptides)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Tissue Extracts)
RN  - 28166-41-8 (alpha-cyano-4-hydroxycinnamate)
RN  - 2968PHW8QP (Citric Acid)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (CSK Tyrosine-Protein Kinase)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - N9BUG430K8 (diammonium hydrogen citrate)
SB  - IM
MH  - Animals
MH  - CSK Tyrosine-Protein Kinase
MH  - Cell Line, Tumor
MH  - Citric Acid/*analogs & derivatives/chemistry
MH  - Coumaric Acids/*chemistry
MH  - Male
MH  - Melanoma/chemistry/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasm Transplantation
MH  - Peptides/analysis/chemistry/*metabolism
MH  - Phosphorylation
MH  - Protein Kinases/metabolism
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Tissue Extracts/chemistry/*metabolism
MH  - src-Family Kinases
EDAT- 2007/09/18 09:00
MHDA- 2007/12/21 09:00
CRDT- 2007/09/18 09:00
PHST- 2006/12/20 00:00 [received]
PHST- 2007/08/02 00:00 [revised]
PHST- 2007/08/05 00:00 [accepted]
PHST- 2007/09/18 09:00 [pubmed]
PHST- 2007/12/21 09:00 [medline]
PHST- 2007/09/18 09:00 [entrez]
AID - S1044-0305(07)00689-7 [pii]
AID - 10.1016/j.jasms.2007.08.002 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2007 Nov;18(11):1925-31. doi: 
      10.1016/j.jasms.2007.08.002. Epub 2007 Aug 15.