PMID- 17870446
OWN - NLM
STAT- MEDLINE
DCOM- 20080508
LR  - 20181201
IS  - 0939-6411 (Print)
IS  - 0939-6411 (Linking)
VI  - 68
IP  - 1
DP  - 2008 Jan
TI  - Direct plasmid DNA encapsulation within PLGA nanospheres by single oil-in-water 
      emulsion method.
PG  - 105-11
AB  - Plasmid DNA was encapsulated within poly(d,l-lactic-co-glycolic acid) (PLGA) 
      nanospheres by using polyethylene glycol (PEG) assisted solubilization technique 
      of plasmid DNA in organic solvents. Plasmid DNA was solubilized in an organic 
      solvent mixture composed of 80% methylene chloride and 20% DMSO by producing 
      PEG/DNA nano-complexes having an average diameter less than 100 nm. DNA could be 
      solubilized in the organic solvent mixture to a greater extent with increasing 
      the weight ratio of PEG/DNA. PLGA nanospheres encapsulating DNA were successfully 
      prepared by the single O/W emulsion method. They exhibited greater loading 
      efficiency and better structural integrity, compared to those prepared by the 
      W/O/W double emulsion method. Plasmid DNA could be successfully delivered to 
      macrophage cells to express an exogenous gene. This new formulation enabled high 
      loading of intact plasmid DNA within PLGA nanospheres useful for DNA vaccines.
FAU - Mok, Hyejung
AU  - Mok H
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology, Daejeon, Republic of Korea.
FAU - Park, Tae Gwan
AU  - Park TG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070718
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Emulsions)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*chemistry
MH  - Emulsions/chemistry
MH  - Gene Expression
MH  - Gene Transfer Techniques
MH  - Genetic Therapy/methods
MH  - Lactic Acid/*chemistry
MH  - Macrophages/metabolism
MH  - Nanospheres
MH  - Particle Size
MH  - Plasmids/*chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Solubility
MH  - Solvents
EDAT- 2007/09/18 09:00
MHDA- 2008/05/09 09:00
CRDT- 2007/09/18 09:00
PHST- 2006/11/29 00:00 [received]
PHST- 2007/04/20 00:00 [revised]
PHST- 2007/04/20 00:00 [accepted]
PHST- 2007/09/18 09:00 [pubmed]
PHST- 2008/05/09 09:00 [medline]
PHST- 2007/09/18 09:00 [entrez]
AID - S0939-6411(07)00257-3 [pii]
AID - 10.1016/j.ejpb.2007.04.022 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2008 Jan;68(1):105-11. doi: 10.1016/j.ejpb.2007.04.022. 
      Epub 2007 Jul 18.