PMID- 17874999
OWN - NLM
STAT- MEDLINE
DCOM- 20071207
LR  - 20211203
IS  - 1474-9718 (Print)
IS  - 1474-9718 (Linking)
VI  - 6
IP  - 5
DP  - 2007 Oct
TI  - Transcriptional response to aging and caloric restriction in heart and adipose 
      tissue.
PG  - 673-88
AB  - Sustained caloric restriction (CR) extends lifespan in animal models but the 
      mechanism and primary tissue target(s) have not been identified. Gene expression 
      changes with aging and CR were examined in both heart and white adipose tissue 
      (WAT) of Fischer 344 (F344) male rats using Affymetrix RAE 230 arrays and 
      validated by quantitative reverse transcriptase-polymerase chain reaction 
      (qRT-PCR) on 18 genes. As expected, age had a substantial effect on transcription 
      on both tissues, although only 21% of cardiac age-associated genes were also 
      altered in WAT. Gene set enrichment analysis revealed coordinated small magnitude 
      changes in ribosomal, proteasomal, and mitochondrial genes with similarities in 
      aging between heart and WAT. CR had very different effects on these two tissues 
      at the transcriptional level. In heart, very few age-associated expression 
      changes were affected by CR, while in WAT, CR suppressed a substantial subset of 
      the age-associated changes. Genes unaltered by aging but altered by CR were 
      identified in WAT but not heart. Most interestingly, we identified a gene 
      expression signature associated with mammalian target of rapamycin (mTOR) 
      activity that was down-regulated with age but preserved by CR in both WAT and 
      heart. In addition, lipid metabolism genes, particularly those associated with 
      peroxisome proliferator-activated receptor gamma (PPARgamma)-mediated 
      adipogenesis were reduced with age but preserved with CR in WAT. These results 
      highlight tissue-specific differences in the gene expression response to CR and 
      support a role for CR-mediated preservation of mTOR activity and adipogenesis in 
      aging WAT.
FAU - Linford, Nancy J
AU  - Linford NJ
AD  - Department of Pathology, University of Washington, Seattle, WA 98195, USA.
FAU - Beyer, Richard P
AU  - Beyer RP
FAU - Gollahon, Katherine
AU  - Gollahon K
FAU - Krajcik, Rozlyn A
AU  - Krajcik RA
FAU - Malloy, Virginia L
AU  - Malloy VL
FAU - Demas, Vasiliki
AU  - Demas V
FAU - Burmer, Glenna C
AU  - Burmer GC
FAU - Rabinovitch, Peter S
AU  - Rabinovitch PS
LA  - eng
GR  - F32 AG021827/AG/NIA NIH HHS/United States
GR  - 4 R44 AG16550/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Aging Cell
JT  - Aging cell
JID - 101130839
RN  - 0 (PPAR gamma)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adipogenesis
MH  - Adipose Tissue, White/*metabolism
MH  - Aging/*genetics
MH  - Animals
MH  - *Caloric Restriction
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Male
MH  - Myocardium/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - PPAR gamma/genetics/metabolism
MH  - Protein Kinases/genetics
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - TOR Serine-Threonine Kinases
MH  - *Transcription, Genetic
EDAT- 2007/09/19 09:00
MHDA- 2007/12/08 09:00
CRDT- 2007/09/19 09:00
PHST- 2007/09/19 09:00 [pubmed]
PHST- 2007/12/08 09:00 [medline]
PHST- 2007/09/19 09:00 [entrez]
AID - ACE319 [pii]
AID - 10.1111/j.1474-9726.2007.00319.x [doi]
PST - ppublish
SO  - Aging Cell. 2007 Oct;6(5):673-88. doi: 10.1111/j.1474-9726.2007.00319.x.