PMID- 17875385
OWN - NLM
STAT- MEDLINE
DCOM- 20080117
LR  - 20111110
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 48
IP  - 3
DP  - 2007 Dec
TI  - Identification of proteins involved in aggregation of human dermal papilla cells 
      by proteomics.
PG  - 189-97
AB  - BACKGROUND: The dermal papilla is a major component of hair, which signals the 
      follicular epithelial cells to prolong the hair growth process. To date, little 
      is known about the significance of the specific protein(s) express in the dermal 
      papilla cells (DPC) with regard to their aggregative behaviour. OBJECTIVES: To 
      identify proteins involved in aggregative behaviour of DPC, we comparatively 
      analyzed the proteome of cells with and without aggregative behaviour. METHODS: A 
      series of methods were used, including two-dimensional gel electrophoresis 
      (2-DE), PDQuest software analysis of 2-DE gels, peptide mass fingerprinting based 
      on matrix-assisted laser desorption/ionisation-time of flight-mass spectrometry 
      (MALDI-TOF-MS), and NCBInr database searching, to separate and identify 
      differentially expressed proteins. Western blotting and reverse transcriptase 
      polymerase chain reaction (RT-PCR) were used to validate the differentially 
      expressed proteins. RESULTS: Image analysis revealed that averages of 618+/-22 
      and 568+/-47 protein spots were detected in passages 3 and 10 DPC, respectively. 
      Twenty-four differential protein spots were measured with MALDI-TOF-MS. A total 
      of 17 spots yielded good spectra, and 15 spots matched with known proteins after 
      database searching. Western blotting confirmed that heat shocking protein 70 was 
      up-regulated in passage 3 DPC. Over-expression of mitochondrial ribosomal protein 
      S7 was confirmed by RT-PCR, indicating that they are involved in aggregation of 
      DPC through some signaling pathway. CONCLUSIONS: The clues provided by the 
      comparative proteome strategy utilized here will shed light on molecular 
      mechanisms of DPC in aggregative behaviour.
FAU - Rushan, Xia
AU  - Rushan X
AD  - Department of Dermatology, Southwest Hospital, Third Military Medical University, 
      Gaotanyan Road, Chongqing 400038, China. xrs4366@sina.com.cn
FAU - Fei, Hao
AU  - Fei H
FAU - Zhirong, Mou
AU  - Zhirong M
FAU - Yu-Zhang, Wu
AU  - Yu-Zhang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070917
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (transgelin)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Blotting, Western
MH  - Cell Aggregation/physiology
MH  - Dermis/*cytology/*physiology
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Female
MH  - HSP70 Heat-Shock Proteins/analysis/*metabolism
MH  - Hair/*cytology/growth & development/*physiology
MH  - Humans
MH  - Male
MH  - Microfilament Proteins/analysis/metabolism
MH  - Molecular Sequence Data
MH  - Muscle Proteins/analysis/metabolism
MH  - Proteomics/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Up-Regulation
EDAT- 2007/09/19 09:00
MHDA- 2008/01/18 09:00
CRDT- 2007/09/19 09:00
PHST- 2007/02/09 00:00 [received]
PHST- 2007/06/10 00:00 [revised]
PHST- 2007/06/20 00:00 [accepted]
PHST- 2007/09/19 09:00 [pubmed]
PHST- 2008/01/18 09:00 [medline]
PHST- 2007/09/19 09:00 [entrez]
AID - S0923-1811(07)00249-6 [pii]
AID - 10.1016/j.jdermsci.2007.06.013 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2007 Dec;48(3):189-97. doi: 10.1016/j.jdermsci.2007.06.013. Epub 
      2007 Sep 17.