PMID- 17875739
OWN - NLM
STAT- MEDLINE
DCOM- 20071127
LR  - 20220317
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 67
IP  - 18
DP  - 2007 Sep 15
TI  - Characterization of viral particles isolated from primary cultures of human 
      breast cancer cells.
PG  - 8960-5
AB  - The association of human breast cancer with sequences similar to the mouse 
      mammary tumor virus (MMTV) has been shown, but convincing evidence for the 
      presence of viral particles in breast tumors has been lacking. We have described 
      the complete proviral structure of a retrovirus in human breast cancer. This 
      provirus, designated as human mammary tumor virus (HMTV), was 95% homologous to 
      MMTV and revealed features of a replication-competent virus. We have therefore 
      investigated the production of viral particles in primary cultures of human 
      breast cancer (MSSM). Cells isolated from ascites or pleural effusions of 
      patients with metastatic breast cancer contained viral sequences in their DNA, 
      expressed Env protein, and showed retroviral particles by electron microscopy. 
      Viral particles from culture media exhibited morphologic features of 
      beta-retroviruses sedimenting at buoyant densities of 1.12 to 1.18 g/mL in 
      sucrose gradients and showed reverse transcriptase activity. cDNA sequences from 
      virion RNA were synthesized, amplified, and sequenced and all the virion genes 
      were detected and 70% of the virion RNA was sequenced. The sequence homologies 
      were, respectively, 85% to 95% compared with the MMTV and HMTV proviruses we have 
      previously described. These results clearly show that breast cancer cells in 
      primary cultures produced HMTV viral particles that are similar to the mouse 
      virus and which may play a role in human breast cancer pathogenesis.
FAU - Melana, Stella M
AU  - Melana SM
AD  - Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, 
      USA.
FAU - Nepomnaschy, Irene
AU  - Nepomnaschy I
FAU - Sakalian, Michael
AU  - Sakalian M
FAU - Abbott, Andrea
AU  - Abbott A
FAU - Hasa, Jennifer
AU  - Hasa J
FAU - Holland, James F
AU  - Holland JF
FAU - Pogo, Beatriz G T
AU  - Pogo BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Breast Neoplasms/*virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Viral/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Virion/*genetics/isolation & purification
EDAT- 2007/09/19 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/09/19 09:00
PHST- 2007/09/19 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/09/19 09:00 [entrez]
AID - 67/18/8960 [pii]
AID - 10.1158/0008-5472.CAN-06-3892 [doi]
PST - ppublish
SO  - Cancer Res. 2007 Sep 15;67(18):8960-5. doi: 10.1158/0008-5472.CAN-06-3892.