PMID- 17877199
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20091119
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 28
IP  - 4
DP  - 2007 Apr
TI  - [Human multiple myeloma cell line RPMI8226 activates brain derived neurotrophic 
      factor autocrine loop of co-cultured endothelial cells].
PG  - 235-8
AB  - OBJECTIVE: To study the influence of multiple myeloma cells on normal endothelial 
      cells in co-culture system. METHODS: Human multiple myeloma cell line RPMI8226 
      was co-cultured with human umbilical vein endothelial cells (HUVECs). HUVECs 
      cultured alone were used as control. The expression of brain derived neurotrophic 
      factor (BDNF) and its specific acceptor TrkB mRNA and protein in HUVECs were 
      determined by RT-PCR and Western blot, respectively, BDNF levels in culture 
      supernatant by enzyme-linked immunosorbent assay (ELISA). After transferring the 
      co-culture, the effects RPMI8226 on HUVECs angiogenesis were studied by modified 
      transwell migration assay and net-like formation assay. RESULTS: The median BDNF 
      concentration in culture supernatant was increased in co-cultured HUVECs compared 
      with that in HUVECs cultured alone [(31.6 +/- 7.2) ng/ml vs (12.4 +/- 5.1) ng/ml, 
      P < 0.05]. The expression of BDNF transcript demonstrated by RT-PCR did the same 
      in the two culture systems (1.7 fold increase, P < 0.05). TrkB mRNA was hardly 
      detected in culture of HUVECs alone but was increased in co-cultured HUVECs (4.4- 
      fold increase, P < 0.05). The BDNF and TrkB protein expressions determined by 
      Western blot were similar to that of their mRNAs. On the other hand, the RPMI8226 
      activated HUVECs showed enhanced migration and net-like formation, being 
      increased by 99% and 72% , respectively. Addition of anti-human BDNF antibody to 
      the culture medium partly reduced these effects. CONCLUSION: Multiple myeloma 
      cells activated BDNF/TrkB autocrine loops in co-cultured endothelial cells and 
      resulted in endothelial self-activating angiogenesis.
FAU - Wang, Ya-Dan
AU  - Wang YD
AD  - Institution of Hematology and Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Hu, Yu
AU  - Hu Y
FAU - Sun, Chun-Yan
AU  - Sun CY
FAU - He, Wen-Juan
AU  - He WJ
FAU - Zhang, Xiao-Ping
AU  - Zhang XP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Communication
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Endothelial Cells/cytology/*metabolism
MH  - Humans
MH  - Multiple Myeloma/*pathology
MH  - Neovascularization, Physiologic
MH  - RNA, Messenger/metabolism
MH  - Receptor, trkB/*metabolism
EDAT- 2007/09/20 09:00
MHDA- 2009/10/01 06:00
CRDT- 2007/09/20 09:00
PHST- 2007/09/20 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2007/09/20 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2007 Apr;28(4):235-8.