PMID- 17877535
OWN - NLM
STAT- MEDLINE
DCOM- 20071207
LR  - 20181113
IS  - 0959-9673 (Print)
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Linking)
VI  - 88
IP  - 5
DP  - 2007 Oct
TI  - Development of an experimental model of brain tissue heterotopia in the lung.
PG  - 337-42
AB  - The presence of heterotopic brain tissue in the lung is a rare abnormality. The 
      cases reported thus far are usually associated with neural tube defects (NTD). As 
      there are no reports of experimental models of NTD that present this abnormality, 
      the objective of the present study was to develop a surgical method of brain 
      tissue heterotopia in the lung. We used 24 pregnant Swiss mice divided into two 
      groups of 12 animals each, denoted 17GD and 18GD according to the gestational day 
      (GD) when caesarean section was performed to collect the fetuses. Surgery was 
      performed on the 15th GD, one fetus was removed by hysterectomy and its brain 
      tissue was cut into small fragments and implanted in the lung of its litter 
      mates. Thirty-four live fetuses were obtained from the 17GD group. Of these, 
      eight (23.5%) were used as control (C), eight (23.5%) were sham operated (S) and 
      18 (52.9%) were used for pulmonary brain tissue implantation (PBI). Thirty live 
      fetuses were obtained from the females of the 18GD group. Of these, eight (26.6%) 
      were C, eight (26.6%) S and 14 (46.6%) were used for PBI. Histological 
      examination of the fetal trunks showed implantation of GFAP-positive brain tissue 
      in 85% of the fetuses of the 17GD group and in 100% of those of the 18GD group, 
      with no significant difference between groups for any of the parameters analysed. 
      The experimental model proved to be efficient and of relatively simple execution, 
      showing complete integration of the brain tissue with pulmonary and pleural 
      tissue and thus representing a model that will permit the study of different 
      aspects of cell implantation and interaction.
FAU - Quemelo, Paulo Roberto Veiga
AU  - Quemelo PR
AD  - Department of Pathology, Ribeirao Preto School of Medicine, University of Sao 
      Paulo, Ribeirao Preto, SP, Brazil.
FAU - Sbragia, Lourenco
AU  - Sbragia L
FAU - Peres, Luiz Cesar
AU  - Peres LC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
SB  - IM
MH  - Animals
MH  - Brain/embryology/*pathology/surgery
MH  - Brain Tissue Transplantation
MH  - Choristoma/*pathology
MH  - Female
MH  - Fetus/*pathology/surgery
MH  - Immunohistochemistry
MH  - *Lung
MH  - Mice
MH  - Mice, Inbred Strains
MH  - *Models, Animal
MH  - Neural Tube Defects/*pathology/surgery
MH  - Pregnancy
PMC - PMC2517333
EDAT- 2007/09/20 09:00
MHDA- 2007/12/08 09:00
PMCR- 2008/10/01
CRDT- 2007/09/20 09:00
PHST- 2007/09/20 09:00 [pubmed]
PHST- 2007/12/08 09:00 [medline]
PHST- 2007/09/20 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - IEP536 [pii]
AID - 10.1111/j.1365-2613.2007.00536.x [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2007 Oct;88(5):337-42. doi: 10.1111/j.1365-2613.2007.00536.x.