PMID- 17878366
OWN - NLM
STAT- MEDLINE
DCOM- 20071106
LR  - 20220330
IS  - 0022-1767 (Print)
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 179
IP  - 7
DP  - 2007 Oct 1
TI  - Kruppel-like factor 4 regulates B cell number and activation-induced B cell 
      proliferation.
PG  - 4679-84
AB  - Kruppel-like factor 4 (Klf4) is a transcription factor and functions in 
      regulating cell differentiation, cell growth, and cell cycle. Although Klf4 is 
      expressed in lymphocytes, its function in lymphocytes is unknown. In this study, 
      we report that the levels of Klf4 expression were low in pro-B cells and 
      continuously increased in pre-B and in mature B cells. Upon activation, Klf4 was 
      rapidly decreased in mature B cells after 2 h of activation. A modest decrease in 
      numbers of pre-B cells in bone marrow and mature B cells in spleen was observed 
      in Klf4-deficient mice. In the absence of Klf4, fewer B cells entered the S phase 
      of the cell cycle and completed cell division in response to the engagement of 
      BCR and/or CD40 in vitro. Furthermore, the delay in entering the cell cycle is 
      associated with decreased expression of cyclin D2 in B cells that lack Klf4 
      expression. We then demonstrated that Klf4 directly bound to the promoter of 
      cyclin D2 and regulated its expression. These findings demonstrate that Klf4 
      regulates B cell number and activation-induced B cell proliferation through 
      directly acting on the promoter of cyclin D2.
FAU - Klaewsongkram, Jettanong
AU  - Klaewsongkram J
AD  - Laboratory of Immunology, National Institute on Aging, National Institutes of 
      Health, Baltimore, MD 21224, USA.
FAU - Yang, Yinhua
AU  - Yang Y
FAU - Golech, Susanne
AU  - Golech S
FAU - Katz, Jonathan
AU  - Katz J
FAU - Kaestner, Klaus H
AU  - Kaestner KH
FAU - Weng, Nan-Ping
AU  - Weng NP
LA  - eng
GR  - Z01 AG000757-10/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Ccnd2 protein, mouse)
RN  - 0 (Cyclin D2)
RN  - 0 (Cyclins)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*cytology/*immunology/metabolism
MH  - Bone Marrow Cells/cytology/immunology/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cyclin D2
MH  - Cyclins/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/deficiency/genetics/*metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - Spleen/cytology/immunology/metabolism
PMC - PMC2262926
MID - NIHMS41126
COIS- Disclosures The authors have no financial conflict of interest.
EDAT- 2007/09/20 09:00
MHDA- 2007/11/07 09:00
PMCR- 2008/03/05
CRDT- 2007/09/20 09:00
PHST- 2007/09/20 09:00 [pubmed]
PHST- 2007/11/07 09:00 [medline]
PHST- 2007/09/20 09:00 [entrez]
PHST- 2008/03/05 00:00 [pmc-release]
AID - 179/7/4679 [pii]
AID - 10.4049/jimmunol.179.7.4679 [doi]
PST - ppublish
SO  - J Immunol. 2007 Oct 1;179(7):4679-84. doi: 10.4049/jimmunol.179.7.4679.