PMID- 17878452
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20200314
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 86
IP  - 10
DP  - 2007 Oct
TI  - Expression of the glucokinase gene in mule duck liver and glucokinase activities 
      in chicken and mule duck livers.
PG  - 2216-20
AB  - The presence of glucokinase (GK), a critical enzyme controlling glucose 
      homeostasis, particularly liver glucose utilization in mammals, has long been a 
      matter of debate in avian species because a number of investigators have failed 
      to detect GK activity in the livers of chickens and several other avian species. 
      In this study, we cloned a partial GK cDNA from mule duck livers and measured 
      GK-like activity in the livers of mule ducks and broiler chickens under 2 
      nutritional states. Liver samples from 5-wk-old meal-fed male broiler chickens 
      (Ross) were obtained from overnight-fasted chickens (BC) and 5 h after an oral 
      saccharose load (6 mL/kg of BW of a 50% saccharose solution) given just before 
      the meal (BS). Liver samples from 15-wk-old mule ducks were collected after an 
      overnight fast (DC) and 12 h after the last overfeeding meal (DO). A partial cDNA 
      ( approximately 600 bp) was obtained from duck livers. It presented 99% identity 
      with chicken partial GK cDNA (gi 44888789) and 82% identity with human GK (gi 
      15967158). Chicken liver weights represented 1.8 and 3.3% of BW, respectively, 
      for BC and BS (n = 8, P < 0.05). Glucokinase and low-Michaelis constant 
      hexokinase (HK) activity levels were similar in BC (respectively, 0.88 and 1.00 
      mU/mg of protein). In response to the meal load, GK activity increased 
      significantly (+57%), whereas HK decreased (-46%) in BS. Duck liver weights 
      represented 1.4 and 7.6% of BW, respectively, for DC and DO (n = 8, P < 0.05). In 
      DC livers, GK activity was significantly higher than HK activity (respectively, 
      1.76 and 0.63 mU/mg of protein). Both activities were significantly increased in 
      DO (2 times, n = 8, P < 0.05). In conclusion, GK is present in ducks as well as 
      chickens, and it is nutritionally regulated in avian species as well as in 
      mammals. Further work will determine whether the higher liver GK activity and 
      GK:HK ratio in DC compared with BC is related to age or BW or linked to the high 
      lipogenic capacity of the duck liver.
FAU - Berradi, H
AU  - Berradi H
AD  - INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France.
FAU - Bernadet, M D
AU  - Bernadet MD
FAU - Guy, G
AU  - Guy G
FAU - Rideau, N
AU  - Rideau N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Insulin)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animal Feed
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Chickens/*genetics/*metabolism
MH  - Diet
MH  - Ducks/*genetics/*metabolism
MH  - Gene Expression Regulation, Enzymologic
MH  - Glucokinase/*genetics/*metabolism
MH  - Insulin/metabolism
MH  - Liver/*enzymology
MH  - Male
EDAT- 2007/09/20 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/09/20 09:00
PHST- 2007/09/20 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/09/20 09:00 [entrez]
AID - S0032-5791(19)39254-5 [pii]
AID - 10.1093/ps/86.10.2216 [doi]
PST - ppublish
SO  - Poult Sci. 2007 Oct;86(10):2216-20. doi: 10.1093/ps/86.10.2216.