PMID- 17879208
OWN - NLM
STAT- MEDLINE
DCOM- 20080122
LR  - 20131121
IS  - 1121-8428 (Print)
IS  - 1121-8428 (Linking)
VI  - 20
IP  - 4
DP  - 2007 Jul-Aug
TI  - Inflammation, malnutrition and vascular contraction in experimental chronic 
      kidney disease.
PG  - 423-9
AB  - BACKGROUND: Inflammation is a well-defined factor influencing the development of 
      cardiovascular complications in chronic renal failure. The aim of this study was 
      to examine systemic inflammatory state defined by the level of serum haptoglobin, 
      local inflammation defined by monocyte chemoattractant protein-1 (MCP-1) level 
      and arterial response to phenylephrine in different stages of renal failure. 
      METHODS: Experiments were performed on male Wistar rats, weighing 290-380 g. The 
      rats were divided into 4 groups: I (control) was shame-operated (n=12); II 
      underwent 1/2 nephrectomy (n=12); III, 3/4 nephrectomy (n=8); IV 5/6 nephrectomy 
      (n=12). After 4 weeks, blood pressure (BP) in carotid artery was measured, and 
      blood was collected for blood urea nitrogen, creatinine, albumin, haptoglobin and 
      MCP-1. We compared the smooth muscle contractility after stimulation of 
      alpha1-adrenoreceptor with phenylephrine in all groups. The constriction of 
      artery was measured as the increase in perfusion pressure at a constant flow of 
      the perfusion fluid. Cumulative response curves (CRCs) were obtained using the 
      van Rossum method. RESULTS: We observed a significant shift of CRCs to the left 
      in group III (calculated half-maximal contraction [EC50] was 1.55 x 10(-7) M/L 
      vs. 7.71 x 10(-7) M/L in control) and a nonsignificant shift of CRCs to the left 
      in group II (3.62 x 10(-7) M/L). Unexpectedly, rat tail arteries from the rats in 
      the 5/6 nephrectomy group were characterized by diminished contraction response 
      to phenylephrine (EC50 9.57 x 10-7 M/L). Systemic inflammation defined by 
      haptoglobin level occurred in the 1/2 nephrectomy group and did not increase in 
      more advanced stages of renal disease. Local inflammation (MCP-1 level) increased 
      together with the renal failure progression. We found a positive correlation 
      between MCP-1 level and haptoglobin only in the 5/6 nephrectomy group (r=0.65; 
      p<0.01). CONCLUSION: The inflammatory state which affects vascular smooth muscle 
      cells plays a key role in determining vascular contraction and resistant artery 
      tone.
FAU - Brymora, Andrzej
AU  - Brymora A
AD  - Department of Nephrology, Hypertension and Internal Diseases, Collegium Medicum 
      in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland. nerka@nerka.cpro.pl
FAU - Flisinski, Mariusz
AU  - Flisinski M
FAU - Grzesk, Grzegorz
AU  - Grzesk G
FAU - Szadujkis-Szadurski, Leszek
AU  - Szadujkis-Szadurski L
FAU - Odrowaz-Sypniewska, Grazyna
AU  - Odrowaz-Sypniewska G
FAU - Manitius, Jacek
AU  - Manitius J
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Nephrol
JT  - Journal of nephrology
JID - 9012268
RN  - 0 (Adrenergic alpha-Agonists)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Haptoglobins)
RN  - 0 (Vasoconstrictor Agents)
RN  - 1WS297W6MV (Phenylephrine)
SB  - IM
MH  - Adrenergic alpha-Agonists/pharmacology
MH  - Animals
MH  - Arteries/drug effects/physiopathology
MH  - Arteriosclerosis/*etiology
MH  - Chemokine CCL2/blood
MH  - Disease Models, Animal
MH  - Haptoglobins/analysis
MH  - *Inflammation
MH  - Kidney Failure, Chronic/*complications/physiopathology
MH  - Male
MH  - *Malnutrition
MH  - Muscle Contraction/drug effects
MH  - Myocytes, Smooth Muscle/drug effects
MH  - Phenylephrine/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - *Vasoconstriction
MH  - Vasoconstrictor Agents/pharmacology
EDAT- 2007/09/20 09:00
MHDA- 2008/01/23 09:00
CRDT- 2007/09/20 09:00
PHST- 2007/09/20 09:00 [pubmed]
PHST- 2008/01/23 09:00 [medline]
PHST- 2007/09/20 09:00 [entrez]
PST - ppublish
SO  - J Nephrol. 2007 Jul-Aug;20(4):423-9.