PMID- 17880937
OWN - NLM
STAT- MEDLINE
DCOM- 20071203
LR  - 20220331
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 311
IP  - 1
DP  - 2007 Nov 1
TI  - Regulation of preimplantation embryo development by brain-derived neurotrophic 
      factor.
PG  - 147-58
AB  - Hormonal factors secreted by embryos and reproductive tracts are important for 
      successful development of preimplantation embryos. We found expression of 
      brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5) 
      transcripts at its highest levels in the blastocyst stages. The transcripts for 
      their receptor, TrkB, were detectable throughout the early embryonic stages with 
      an increase after the early blastocyst stage. Both BDNF and TrkB are expressed in 
      trophectoderm cells, whereas ligand-binding studies indicated specific binding of 
      BDNF to trophectoderm cells. Furthermore, BDNF and NT-4/5 were produced in 
      pregnant oviducts and uteri. Treatment with BDNF promoted the development of 
      two-cell-stage embryos into blastocysts showing increased proliferation and 
      decreased apoptosis. The effects of BDNF were blocked by the TrkB ectodomain or a 
      Trk receptor inhibitor, K252a. Studies using specific inhibitors demonstrated the 
      roles of the PI3K, but not the ERK, pathway in mediating BDNF actions. Under 
      high-density embryo cultures, treatment with the TrkB ectodomain or K252a alone 
      also inhibited embryonic development and survival, suggesting potential autocrine 
      actions of BDNF produced by the embryo. In vivo experiments further demonstrated 
      that K252a treatment suppressed early embryo development by inhibiting blastocyst 
      cell numbers, and increasing blastocyst apoptosis. Our findings suggested that 
      BDNF signaling plays important paracrine roles during blastocyst development by 
      promoting the development of preimplantation embryos.
FAU - Kawamura, Kazuhiro
AU  - Kawamura K
AD  - Department of Obstetrics and Gynecology, Akita University School of Medicine, 
      Akita, 010-8543, Japan. kawamura@yf7.so-net.ne.jp
FAU - Kawamura, Nanami
AU  - Kawamura N
FAU - Fukuda, Jun
AU  - Fukuda J
FAU - Kumagai, Jin
AU  - Kumagai J
FAU - Hsueh, Aaron J W
AU  - Hsueh AJ
FAU - Tanaka, Toshinobu
AU  - Tanaka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070821
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Embryo, Mammalian/*metabolism
MH  - Female
MH  - Mice
MH  - Nerve Growth Factors/metabolism
MH  - Oviducts/metabolism
MH  - Receptor, trkB/metabolism
MH  - Signal Transduction
MH  - Uterus/metabolism
EDAT- 2007/09/21 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/09/21 09:00
PHST- 2007/06/15 00:00 [received]
PHST- 2007/07/17 00:00 [revised]
PHST- 2007/08/09 00:00 [accepted]
PHST- 2007/09/21 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/09/21 09:00 [entrez]
AID - S0012-1606(07)01271-7 [pii]
AID - 10.1016/j.ydbio.2007.08.026 [doi]
PST - ppublish
SO  - Dev Biol. 2007 Nov 1;311(1):147-58. doi: 10.1016/j.ydbio.2007.08.026. Epub 2007 
      Aug 21.