PMID- 17881205
OWN - NLM
STAT- MEDLINE
DCOM- 20080201
LR  - 20151119
IS  - 0940-2993 (Print)
IS  - 0940-2993 (Linking)
VI  - 59
IP  - 3-4
DP  - 2007 Nov
TI  - Nicotine-induced prenatal overexposure to maternal glucocorticoid and 
      intrauterine growth retardation in rat.
PG  - 245-51
AB  - Overexposure to glucocorticoid during fetal development can result in 
      intrauterine growth retardation (IUGR) as well as other diseases after birth. The 
      purpose of this study is to investigate the possibility of glucocorticoid 
      disturbance-mediated nicotine-induced IUGR after chronic prenatal exposure. 
      Nicotine at 1.0mg/kg twice a day was administered subcutaneously to pregnant rats 
      from gestational day (GD) 8 to GD 15 (mid-gestation) or GD 21 (late-gestation). 
      Placental weights and fetal developmental parameters were recorded. 
      Corticosterone levels were determined by radioimmunoassay. The mRNA expressions 
      of adrenal steroidogenic acute regulatory protein (StAR), cytochrome P450 
      cholesterol side chain cleavage (P450scc) and placental 11 beta-hydroxysteroid 
      dehydrogenase type 2 (11 beta-HSD-2) were determined using real-time quantitative 
      RT-PCR. The results showed that prenatal chronic nicotine exposure causes IUGR in 
      rats (P<0.01); in response to nicotine exposure, maternal serum corticosterone 
      levels were elevated at mid- and late-gestations (P<0.05); mRNA expressions of 
      StAR and P450scc increased in maternal adrenals (P<0.05 or 0.01) but decreased in 
      fetal adrenals (P=0.16 or 0.11). Furthermore, the mRNA levels of placental 11 
      beta-HSD-2 were reduced at mid- and late-gestations (P<0.05). These results 
      suggest that nicotine-induced IUGR is associated with the disturbances of 
      glucocorticoid homeostasis in maternal and fetal rats. A possible underlying 
      mechanism is that long term nicotine administration leads to fetal overexposure 
      to maternal glucocorticoid by the combined effect of increased maternal 
      glucocorticoid level and impaired placental barrier to it, all of which 
      eventually leads to the fetal adrenocortical dysfunction and IUGR.
FAU - Chen, Man
AU  - Chen M
AD  - Department of Pharmacology, Basic Medical School of Wuhan University, Donghu Road 
      115(#) Hubei Province Wuhan 430071, China.
FAU - Wang, Ting
AU  - Wang T
FAU - Liao, Zhang-Xiu
AU  - Liao ZX
FAU - Pan, Xiao-Liang
AU  - Pan XL
FAU - Feng, Ying-Hong
AU  - Feng YH
FAU - Wang, Hui
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070919
PL  - Germany
TA  - Exp Toxicol Pathol
JT  - Experimental and toxicologic pathology : official journal of the Gesellschaft fur 
      Toxikologische Pathologie
JID - 9208920
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 2)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenase Type 2/genetics/metabolism
MH  - Adrenal Glands/drug effects/embryology/metabolism
MH  - Animals
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics/metabolism
MH  - Corticosterone/*blood
MH  - Disease Models, Animal
MH  - Female
MH  - Fetal Development/drug effects
MH  - Fetal Growth Retardation/*chemically induced/pathology
MH  - Fetal Weight/drug effects
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Injections, Subcutaneous
MH  - *Maternal Exposure
MH  - Maternal-Fetal Exchange/*drug effects/physiology
MH  - Nicotine/*toxicity
MH  - Nicotinic Agonists/*toxicity
MH  - Organ Size/drug effects
MH  - Phosphoproteins/genetics/metabolism
MH  - Placenta/drug effects/enzymology/pathology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Specific Pathogen-Free Organisms
EDAT- 2007/09/21 09:00
MHDA- 2008/02/02 09:00
CRDT- 2007/09/21 09:00
PHST- 2007/03/12 00:00 [received]
PHST- 2007/05/11 00:00 [accepted]
PHST- 2007/09/21 09:00 [pubmed]
PHST- 2008/02/02 09:00 [medline]
PHST- 2007/09/21 09:00 [entrez]
AID - S0940-2993(07)00039-5 [pii]
AID - 10.1016/j.etp.2007.05.007 [doi]
PST - ppublish
SO  - Exp Toxicol Pathol. 2007 Nov;59(3-4):245-51. doi: 10.1016/j.etp.2007.05.007. Epub 
      2007 Sep 19.