PMID- 17881265
OWN - NLM
STAT- MEDLINE
DCOM- 20080618
LR  - 20080304
IS  - 1090-3798 (Print)
IS  - 1090-3798 (Linking)
VI  - 12
IP  - 2
DP  - 2008 Mar
TI  - Neurotrophic factor expression in newborns with myelomeningocele: preliminary 
      data.
PG  - 113-8
AB  - BACKGROUND: Neurotrophic factors play a crucial role in the stimulation of 
      sprouting, synaptic plasticity and reorganization after spinal cord damage. The 
      aim of this study was to investigate the expression of some neurotrophic factors 
      [brain derived neurotrophic factor (BDNF), glial derived neurotrophic factor 
      (GDNF), and nerve growth factor (NGF)] in the cerebrospinal fluid (CSF) of 
      newborns with myelomeningocele (MMC) and to determine their correlations with 
      this malformation. METHODS: To measure the expression of BDNF, GDNF, and NGF, we 
      collected CSF samples of six newborns during the neurosurgical operation to 
      correct the open MMC and of 10 matched controls. Endogenous neurotrophic factor 
      levels were quantified using a two-site immuno-enzymatic assay. The statistical 
      analysis was performed using the Mann-Whitney two-tailed two-sample test. 
      FINDINGS: In the CSF of patients analysis of neurotrophic factor expression 
      showed a significant increase of BDNF, GDNF, and NGF compared to the mean level 
      of the control group (445.8+/-82.3, 86.5+/-2.6, and 59.9+/-6.2 pg/mL, 
      respectively, respect to 10.2+/-5.9, 19.9+/-11.3, and 15.3+/-2.6 pg/mL) 
      (p<0.001). INTERPRETATION: Our study shows an over-expression of neurotrophic 
      factors in the CSF of newborns with MMC. This neurotrophin up-regulation may 
      stimulate axonal sprouting and synaptic reorganization of the damaged neural 
      cells at the site of spinal cord lesion. The neurotrophic factor up-regulation 
      may represent a particularly important biochemical markers of spinal cord damage 
      and might be associated with the severity of spine injury in MMC patients.
FAU - Chiaretti, A
AU  - Chiaretti A
AD  - Department of Paediatric Science, Catholic University Medical School, Rome, 
      Italy.
FAU - Ausili, E
AU  - Ausili E
FAU - Di Rocco, C
AU  - Di Rocco C
FAU - Antonelli, A
AU  - Antonelli A
FAU - Tabacco, F
AU  - Tabacco F
FAU - Focarelli, B
AU  - Focarelli B
FAU - Rendeli, C
AU  - Rendeli C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070918
PL  - England
TA  - Eur J Paediatr Neurol
JT  - European journal of paediatric neurology : EJPN : official journal of the 
      European Paediatric Neurology Society
JID - 9715169
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Axons/physiology
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/cerebrospinal fluid
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor/biosynthesis/cerebrospinal fluid
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Infant, Newborn/*metabolism
MH  - Male
MH  - Meningomyelocele/*metabolism
MH  - Nerve Growth Factors/*biosynthesis/cerebrospinal fluid
EDAT- 2007/09/21 09:00
MHDA- 2008/06/19 09:00
CRDT- 2007/09/21 09:00
PHST- 2007/03/22 00:00 [received]
PHST- 2007/07/12 00:00 [revised]
PHST- 2007/07/12 00:00 [accepted]
PHST- 2007/09/21 09:00 [pubmed]
PHST- 2008/06/19 09:00 [medline]
PHST- 2007/09/21 09:00 [entrez]
AID - S1090-3798(07)00118-3 [pii]
AID - 10.1016/j.ejpn.2007.07.003 [doi]
PST - ppublish
SO  - Eur J Paediatr Neurol. 2008 Mar;12(2):113-8. doi: 10.1016/j.ejpn.2007.07.003. 
      Epub 2007 Sep 18.