PMID- 17881451
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20220311
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 23
DP  - 2007 Dec
TI  - In vivo changes in the patterns of chromatin structure associated with the latent 
      herpes simplex virus type 1 genome in mouse trigeminal ganglia can be detected at 
      early times after butyrate treatment.
PG  - 13248-53
AB  - During herpes simplex virus type 1 (HSV-1) latency in mouse dorsal root ganglia 
      (DRG), chromatin associated with the latency-associated transcript (LAT) region 
      of the viral genome is hyperacetylated at lysines 9 and 14 of histone 3 [H3(K9, 
      K14)], while lytic genes are hypoacetylated. Explanted DRG exhibit a pattern of 
      deacetylation of the LAT enhancer followed by acetylation of the ICP0 promoter at 
      early times postexplant. Recently, we reported that sodium butyrate induced in 
      vivo reactivation of HSV-1 in latent mice. In this study, we assessed the effect 
      of sodium butyrate on the chromatin patterns of latent and butyrate-treated mouse 
      trigeminal ganglia (TG) via chromatin immunoprecipitation (ChIP). We detected 
      deacetylation of acetyl H3(K9, K14) of the LAT promoter and LAT enhancer regions 
      as early as 0.5 h post-butyrate treatment, and this deacetylation corresponded to 
      an increase in the acetylation of the lytic promoters ICP0 and ICP4 at 0.5 h and 
      1 h post-butyrate treatment, respectively. This is the first study to combine in 
      vivo reactivation with the examination of the HSV-1 genome through ChIP assays at 
      early times after the introduction of in vivo reactivation stimuli.
FAU - Neumann, Donna M
AU  - Neumann DM
AD  - Department of Ophthalmology (LSU Eye Center of Excellence), Louisiana State 
      University Health Sciences Center, New Orleans, Louisiana 70112, USA.
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
FAU - Giordani, Nicole V
AU  - Giordani NV
FAU - Bloom, David C
AU  - Bloom DC
FAU - Hill, James M
AU  - Hill JM
LA  - eng
GR  - R01 EY006311/EY/NEI NIH HHS/United States
GR  - F32 EY016316/EY/NEI NIH HHS/United States
GR  - R01 AI048633/AI/NIAID NIH HHS/United States
GR  - F32EY016316/EY/NEI NIH HHS/United States
GR  - P30 EY002377/EY/NEI NIH HHS/United States
GR  - AI07110/AI/NIAID NIH HHS/United States
GR  - AI48633/AI/NIAID NIH HHS/United States
GR  - P30EY002377/EY/NEI NIH HHS/United States
GR  - R01EY006311/EY/NEI NIH HHS/United States
GR  - T32 AI007110/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070919
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Butyrates)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Viral)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Histones)
SB  - IM
MH  - Animals
MH  - Butyrates/*pharmacology
MH  - Chromatin/metabolism
MH  - Chromatin Assembly and Disassembly/*drug effects
MH  - Chromatin Immunoprecipitation
MH  - DNA, Viral/*metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Female
MH  - Herpesvirus 1, Human/genetics/*physiology
MH  - Histone Deacetylase Inhibitors
MH  - Histones/metabolism
MH  - Mice
MH  - Promoter Regions, Genetic
MH  - Trigeminal Ganglion/*virology
MH  - Virus Activation/genetics/*physiology
PMC - PMC2169074
EDAT- 2007/09/21 09:00
MHDA- 2007/12/19 09:00
PMCR- 2008/04/01
CRDT- 2007/09/21 09:00
PHST- 2007/09/21 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/09/21 09:00 [entrez]
PHST- 2008/04/01 00:00 [pmc-release]
AID - JVI.01569-07 [pii]
AID - 1569-07 [pii]
AID - 10.1128/JVI.01569-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Dec;81(23):13248-53. doi: 10.1128/JVI.01569-07. Epub 2007 Sep 19.