PMID- 17881526
OWN - NLM
STAT- MEDLINE
DCOM- 20071011
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 27
IP  - 38
DP  - 2007 Sep 19
TI  - Monoamine oxidase-B mediates ecstasy-induced neurotoxic effects to adolescent rat 
      brain mitochondria.
PG  - 10203-10
AB  - 3,4-Methylenedioxymethamphetamine (MDMA)-induced neurotoxicity and the protective 
      role of monoamine oxidase-B (MAO-B) inhibition were evaluated at the 
      mitochondrial level in various regions of the adolescent rat brain. Four groups 
      of adolescent male Wistar rats were used: (1) saline control, (2) exposed to MDMA 
      (4 x 10 mg/kg, i.p.; two hourly), (3) treated with selegiline (2 mg/kg, i.p.) 30 
      min before the same dosing of MDMA, and (4) treated with selegiline (2 mg/kg, 
      i.p.). Body temperatures were monitored throughout the whole experiment. Animals 
      were killed 2 weeks later, and mitochondria were isolated from several brain 
      regions. Our results showed that "binge" MDMA administration causes, along with 
      sustained hyperthermia, long-term alterations in brain mitochondria as evidenced 
      by increased levels of lipid peroxides and protein carbonyls. Additionally, 
      analysis of mitochondrial DNA (mtDNA) revealed that NDI nicotinamide adenine 
      dinucleotide phosphate dehydrogenase subunit I and NDII (nicotinamide adenine 
      dinucleotide phosphate dehydrogenase subunit II) subunits of mitochondrial 
      complex I and cytochrome c oxidase subunit I of complex IV suffered deletions in 
      MDMA-exposed animals. Inhibition of MAO-B by selegiline did not reduce 
      hyperthermia but reversed MDMA-induced effects in the oxidative stress markers, 
      mtDNA, and related protein expression. These results indicate that monoamine 
      oxidation by MAO-B with subsequent mitochondrial damage may be an important 
      contributing factor for MDMA-induced neurotoxicity.
FAU - Alves, Ema
AU  - Alves E
AD  - Neurobehaviour Unit, Instituto de Biologia Molecular e Celular, Medical School of 
      Porto, University of Porto, 4099-002 Porto, Portugal.
FAU - Summavielle, Teresa
AU  - Summavielle T
FAU - Alves, Cecilia Juliana
AU  - Alves CJ
FAU - Gomes-da-Silva, Joana
AU  - Gomes-da-Silva J
FAU - Barata, Jose Custodio
AU  - Barata JC
FAU - Fernandes, Eduarda
AU  - Fernandes E
FAU - Bastos, Maria de Lourdes
AU  - Bastos Mde L
FAU - Tavares, Maria Amelia
AU  - Tavares MA
FAU - Carvalho, Felix
AU  - Carvalho F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Monoamine Oxidase Inhibitors)
RN  - EC 1.4.3.4 (Monoamine Oxidase)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Brain/drug effects/*enzymology
MH  - Lipid Peroxidation/drug effects/physiology
MH  - Male
MH  - Mitochondria/drug effects/*enzymology
MH  - Monoamine Oxidase/*metabolism
MH  - Monoamine Oxidase Inhibitors/pharmacology
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*toxicity
MH  - Neurotoxicity Syndromes/enzymology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6672671
EDAT- 2007/09/21 09:00
MHDA- 2007/10/12 09:00
PMCR- 2008/03/19
CRDT- 2007/09/21 09:00
PHST- 2007/09/21 09:00 [pubmed]
PHST- 2007/10/12 09:00 [medline]
PHST- 2007/09/21 09:00 [entrez]
PHST- 2008/03/19 00:00 [pmc-release]
AID - 27/38/10203 [pii]
AID - 3258663 [pii]
AID - 10.1523/JNEUROSCI.2645-07.2007 [doi]
PST - ppublish
SO  - J Neurosci. 2007 Sep 19;27(38):10203-10. doi: 10.1523/JNEUROSCI.2645-07.2007.