PMID- 17885211
OWN - NLM
STAT- MEDLINE
DCOM- 20071211
LR  - 20121115
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 27
IP  - 12
DP  - 2007 Dec
TI  - Local delivery of anti-monocyte chemoattractant protein-1 by gene-eluting stents 
      attenuates in-stent stenosis in rabbits and monkeys.
PG  - 2563-8
AB  - OBJECTIVE: We have previously shown that the intramuscular transfer of the 
      anti-monocyte chemoattractant protein-1 (MCP-1) gene (called 7ND) is able to 
      prevent experimental restenosis. The aim of this study was to determine the in 
      vivo efficacy and safety of local delivery of 7ND gene via the gene-eluting stent 
      in reducing in-stent neointima formation in rabbits and in cynomolgus monkeys. 
      METHODS AND RESULTS: We here found that in vitro, 7ND effectively inhibited the 
      chemotaxis of mononuclear leukocytes and also inhibited the 
      proliferation/migration of vascular smooth muscle cells. We then coated stents 
      with a biocompatible polymer containing a plasmid bearing the 7ND gene, and 
      deployed these stents in the iliac arteries of rabbits and monkeys. 7ND 
      gene-eluting stents attenuated stent-associated monocyte infiltration and 
      neointima formation after one month in rabbits, and showed long-term inhibitory 
      effects on neointima formation when assessments were carried out at 1, 3, and 6 
      months in monkeys. CONCLUSIONS: Strategy of inhibiting the action of MCP-1 with a 
      7ND gene-eluting stent reduced in-stent neointima formation with no evidence of 
      adverse effects in rabbits and monkeys. The 7ND gene-eluting stent could be a 
      promising therapy for treatment of restenosis in humans.
FAU - Egashira, Kensuke
AU  - Egashira K
AD  - Department of Cardiovascular Medicine, Graduate School of Medical Science, Kyushu 
      University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. 
      egashira@cardiol.med.kyushu-u.ac.jp
FAU - Nakano, Kaku
AU  - Nakano K
FAU - Ohtani, Kisho
AU  - Ohtani K
FAU - Funakoshi, Kouta
AU  - Funakoshi K
FAU - Zhao, Gang
AU  - Zhao G
FAU - Ihara, Yoshiko
AU  - Ihara Y
FAU - Koga, Jun-Ichiro
AU  - Koga J
FAU - Kimura, Satoshi
AU  - Kimura S
FAU - Tominaga, Ryuji
AU  - Tominaga R
FAU - Sunagawa, Kenji
AU  - Sunagawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070920
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Biocompatible Materials)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Metals)
SB  - IM
MH  - Animals
MH  - Arterial Occlusive Diseases/etiology/genetics/metabolism/pathology/*prevention & 
      control
MH  - Biocompatible Materials
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chemotaxis, Leukocyte
MH  - Constriction, Pathologic/prevention & control
MH  - Disease Models, Animal
MH  - Feasibility Studies
MH  - *Gene Transfer Techniques
MH  - Genetic Therapy/*methods
MH  - Humans
MH  - Iliac Artery/pathology/*surgery
MH  - Kinetics
MH  - Leukocytes, Mononuclear/pathology
MH  - Macaca fascicularis
MH  - Macrophages/pathology
MH  - Male
MH  - Metals
MH  - Mutation
MH  - Plasmids/metabolism
MH  - Prosthesis Design
MH  - Rabbits
MH  - *Stents
MH  - Time Factors
MH  - Tunica Intima/pathology
MH  - Vascular Surgical Procedures/adverse effects/*instrumentation
EDAT- 2007/09/22 09:00
MHDA- 2007/12/12 09:00
CRDT- 2007/09/22 09:00
PHST- 2007/09/22 09:00 [pubmed]
PHST- 2007/12/12 09:00 [medline]
PHST- 2007/09/22 09:00 [entrez]
AID - ATVBAHA.107.154609 [pii]
AID - 10.1161/ATVBAHA.107.154609 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2563-8. doi: 
      10.1161/ATVBAHA.107.154609. Epub 2007 Sep 20.