PMID- 17886100
OWN - NLM
STAT- MEDLINE
DCOM- 20071226
LR  - 20131121
IS  - 0031-3025 (Print)
IS  - 0031-3025 (Linking)
VI  - 39
IP  - 5
DP  - 2007 Oct
TI  - An experimental study on the effects of ethanol and folic acid deficiency, alone 
      or in combination, on pregnant Swiss mice.
PG  - 495-503
AB  - INTRODUCTION: Ethanol is teratogenic, interferes with folic acid and is 
      extensively used by young women. Our objective was to determine the effects of 
      ethanol and/or folate deficiency on mouse fetuses. METHOD: In Experiment 1, 
      pregnant mice receiving a commercial diet were divided into three groups: control 
      (C), low ethanol dose (LE, 0.4 g/kg), and high ethanol dose (HE, 4.0 g/kg). In 
      Experiment 2, pregnant mice receiving a folate-free diet (FFD) were divided into 
      three groups: folate deficiency (FD), folate deficiency plus a low ethanol dose 
      (FDLE), and folate deficiency plus a high ethanol dose (FDHE). Groups C and FD 
      received saline and the remaining groups received ethanol administered i.p. from 
      the 7th to the 9th gestational day (GD) and were sacrificed on the 18th GD. 
      RESULTS: In Experiment 1, Group HE presented congenital anomalies, late fetal 
      death (LFD), lower fetal length and weight and placental weight and diameter than 
      Groups C and LE. In Experiment 2, there was a smaller number of live fetuses, a 
      larger number of reabsorptions and LFD, a smaller length and lower fetal weight, 
      placental weight and diameter in Groups FDLE and FDHE than in Group FD. 
      CONCLUSION: In animals receiving a commercial diet, a high ethanol dose is 
      deleterious to the pregnancy, inducing congenital anomalies, intrauterine growth 
      restriction, reduction of the placenta and increased LFD, events that did not 
      occur with the low dose. However, with a folate free diet, a low ethanol dose is 
      as deleterious as a high dose.
FAU - Gutierrez, Cristiane Minot
AU  - Gutierrez CM
AD  - Department of Pathology, Laboratory of Pediatric Pathology, Faculty of Medicine 
      of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
FAU - Ribeiro, Camila Nunes de Morais
AU  - Ribeiro CN
FAU - de Lima, Giovanna Arcaro
AU  - de Lima GA
FAU - Yanaguita, Maira Yuli
AU  - Yanaguita MY
FAU - Peres, Luiz Cesar
AU  - Peres LC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pathology
JT  - Pathology
JID - 0175411
RN  - 0 (Central Nervous System Depressants)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Central Nervous System Depressants/*toxicity
MH  - Ethanol/*toxicity
MH  - Female
MH  - Fetal Growth Retardation/*chemically induced
MH  - Fetus/*drug effects
MH  - Folic Acid Deficiency/*physiopathology
MH  - Mice
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*physiopathology
EDAT- 2007/09/22 09:00
MHDA- 2007/12/27 09:00
CRDT- 2007/09/22 09:00
PHST- 2007/09/22 09:00 [pubmed]
PHST- 2007/12/27 09:00 [medline]
PHST- 2007/09/22 09:00 [entrez]
AID - 782039022 [pii]
AID - 10.1080/00313020701449290 [doi]
PST - ppublish
SO  - Pathology. 2007 Oct;39(5):495-503. doi: 10.1080/00313020701449290.