PMID- 17886720
OWN - NLM
STAT- MEDLINE
DCOM- 20071017
LR  - 20161021
IS  - 1000-8721 (Print)
IS  - 1000-8721 (Linking)
VI  - 23
IP  - 1
DP  - 2007 Jan
TI  - [Studies on porcine reproductive and respiratory syndrome virus morphogenesis in 
      Marc-145 cell and the ultrastructural changes of the infected cells].
PG  - 46-50
AB  - Electron microscopy was employed for ultrastructural observation of Marc-145 
      cells infected with porcine reproductive and respiratory syndrome virus (PRRSV) 
      SC1 strain and studied the virus morphogenesis in infected cells. The results 
      demonstrated that PRRSV was spherical and enveloped. The virion is 45-65 nm in 
      diameter and its nucleocapsid was approximately 25-30 nm. PRRSV entered Marc-145 
      cells by endocytosis, and replicated in the cytoplasm. The mature viruses were 
      released from infected cells by budding or exocytosis. The main ultrastructural 
      changes of the infected cells were as follows: increased number of cytoplasmic 
      vacuoles, dilated endoplasmic reticulum, mitochondria underwent hyperplasia with 
      its ridges swollen, sloughed, and eventually vacuolated. Typical apoptosis was 
      also observed in the infected Marc-145 cells, which included microvilli sloughing 
      off the cell, appearance of apoptotic bodies and cell fragmentation.
FAU - Wang, Ming-Shu
AU  - Wang MS
AD  - College of Animal Science & Veterinary Medicine, Sichuan Agricultural University, 
      Yaan 625014, Sichuan, China.
FAU - Cheng, An-Chun
AU  - Cheng AC
FAU - Liu, Wu-Mei
AU  - Liu WM
FAU - Zhou, Yi
AU  - Zhou Y
FAU - Guo, Yu-Fei
AU  - Guo YF
FAU - Yuan, Gui-Ping
AU  - Yuan GP
FAU - Chen, Xiao-Yue
AU  - Chen XY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Bing Du Xue Bao
JT  - Bing du xue bao = Chinese journal of virology
JID - 8803009
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Line
MH  - Microscopy, Electron, Transmission
MH  - Porcine respiratory and reproductive syndrome virus/growth & 
      development/*ultrastructure
MH  - Virion/growth & development/ultrastructure
MH  - *Virus Replication
EDAT- 2007/09/25 09:00
MHDA- 2007/10/18 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2007/10/18 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
PST - ppublish
SO  - Bing Du Xue Bao. 2007 Jan;23(1):46-50.