PMID- 17889478
OWN - NLM
STAT- MEDLINE
DCOM- 20080131
LR  - 20131121
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 45
IP  - 5
DP  - 2007 Dec 21
TI  - Simultaneous determination of flavonoids in Ixeridium gracile by micellar 
      electrokinetic chromatography.
PG  - 742-6
AB  - A micellar electrokinetic chromatography (MEKC) method has been developed for the 
      quantitative analysis of five flavonoids: luteolin 7-O-glucoside (LG), 
      2',4'-dihydroxy-dihydrochalcone (DD), 2',4'-dihydroxy-chalcone (DC), 
      7-hydroxy-flavanone (HF) and quercetin 3-O-galactoside (QG) in Ixeridium gracile 
      with UV detection at 275 nm. The applied voltage was 25 kV and the capillary 
      temperature was kept constant at 25 degrees C. The effects of buffer pH, the 
      concentration of electrolyte and organic modifier on migration behavior were 
      studied. Optimum separation condition was achieved with 15 mM borate, 30 mM 
      sodium dodecyl sulfate (SDS) and 10% (v/v) ethanol at pH 10.5. Regression 
      equations showed good linear relationships (correlation coefficients: 0.9984, 
      0.9991, 0.9994, 0.9995 and 0.9997) between the peak area of each compound and 
      their concentrations. The relative standard deviations (R.S.D.) of the migration 
      time and peak area were less 1.67 and 3.53% (intra-day), and 1.82 and 3.73% 
      (inter-day), respectively, under the optimum separation conditions. The contents 
      of the five active compounds in I. gracile were determined with satisfactory 
      repeatability and recovery.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of 
      Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
FAU - Chen, Juan
AU  - Chen J
FAU - Ma, Xue-Mei
AU  - Ma XM
FAU - Shi, Yan-Ping
AU  - Shi YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070819
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Boric Acids)
RN  - 0 (Buffers)
RN  - 0 (Flavonoids)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
SB  - IM
MH  - Asteraceae/*chemistry
MH  - Boric Acids/chemistry
MH  - Buffers
MH  - Calibration
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Flavonoids/*analysis/chemistry/isolation & purification
MH  - Hydrogen-Ion Concentration
MH  - Medicine, Tibetan Traditional
MH  - Molecular Structure
MH  - Regression Analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sodium Dodecyl Sulfate/chemistry
MH  - Spectrophotometry, Ultraviolet
MH  - Temperature
EDAT- 2007/09/25 09:00
MHDA- 2008/02/01 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/04/22 00:00 [received]
PHST- 2007/08/14 00:00 [revised]
PHST- 2007/08/15 00:00 [accepted]
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2008/02/01 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
AID - S0731-7085(07)00477-3 [pii]
AID - 10.1016/j.jpba.2007.08.014 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2007 Dec 21;45(5):742-6. doi: 10.1016/j.jpba.2007.08.014. 
      Epub 2007 Aug 19.