PMID- 17889492
OWN - NLM
STAT- MEDLINE
DCOM- 20080410
LR  - 20071217
IS  - 0732-8893 (Print)
IS  - 0732-8893 (Linking)
VI  - 60
IP  - 1
DP  - 2008 Jan
TI  - A view on Bartonella quintana endocarditis--confirming the molecular diagnosis by 
      specific fluorescence in situ hybridization.
PG  - 99-103
AB  - Culture-negative endocarditis is a frequent problem in cardiology, especially if 
      caused by fastidious organisms. Among these, the diagnostic tools for the 
      detection of Bartonella quintana are still unsatisfactory. In a culture-negative 
      case of suspected endocarditis undergoing aortic valve replacement, polymerase 
      chain reaction amplification and sequencing of the 16S rRNA gene indicated B. 
      quintana infection. To develop a new diagnostic tool, independent from culture 
      and amplification techniques, we designed and optimized an oligonucleotide 
      fluorescence in situ hybridization (FISH) probe specific for B. quintana and 
      suitable for FISH. FISH succeeded in simultaneous visualization and 
      identification of vital microorganisms directly within the aortic valve tissue 
      and in fast and univocal diagnosis of B. quintana endocarditis.
FAU - Gescher, Dorothee Maria
AU  - Gescher DM
AD  - Institut fur Mikrobiologie und Hygiene, Charite-Universitatsmedizin Berlin, 
      D-10117 Berlin, Germany.
FAU - Mallmann, Christian
AU  - Mallmann C
FAU - Kovacevic, Dragoljub
AU  - Kovacevic D
FAU - Schmiedel, Dinah
AU  - Schmiedel D
FAU - Borges, Adrian C
AU  - Borges AC
FAU - Schweickert, Birgitta
AU  - Schweickert B
FAU - Gobel, Ulf B
AU  - Gobel UB
FAU - Moter, Annette
AU  - Moter A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070921
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aortic Valve/microbiology
MH  - Bartonella quintana/genetics/*isolation & purification
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Endocarditis, Bacterial/*diagnosis/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Trench Fever/*diagnosis/*microbiology
EDAT- 2007/09/25 09:00
MHDA- 2008/04/11 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/03/01 00:00 [received]
PHST- 2007/07/18 00:00 [revised]
PHST- 2007/07/20 00:00 [accepted]
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2008/04/11 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
AID - S0732-8893(07)00333-1 [pii]
AID - 10.1016/j.diagmicrobio.2007.07.012 [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 2008 Jan;60(1):99-103. doi: 
      10.1016/j.diagmicrobio.2007.07.012. Epub 2007 Sep 21.