PMID- 17889539
OWN - NLM
STAT- MEDLINE
DCOM- 20080502
LR  - 20191210
IS  - 0960-9776 (Print)
IS  - 0960-9776 (Linking)
VI  - 17
IP  - 1
DP  - 2008 Feb
TI  - Evaluation of FISH image analysis system on assessing HER2 amplification in 
      breast carcinoma cases.
PG  - 80-4
AB  - HER2-positive breast cancer is characterized by aggressive growth and poor 
      prognosis. Women with metastatic breast cancer with over-expression of HER2 
      protein or excessive presence of HER2 gene copies are potential candidates for 
      Herceptin (Trastuzumab) targeted treatment that binds to HER2 receptors on tumor 
      cells and inhibits tumor cell growth. Fluorescence in situ hybridization (FISH) 
      is one of the most widely used methods to determine HER2 status. Typically, 
      evaluation of FISH images involves manual counting of FISH signals in multiple 
      images, a time consuming and error prone procedure. Recently, we developed novel 
      software for the automated evaluation of FISH images and, in this study, we 
      present the first testing of this software on images from two separate research 
      clinics. To our knowledge, this is the first concurrent evaluation of any FISH 
      image analysis software in two different clinics. The evaluation shows that the 
      developed FISH image analysis software can accelerate evaluation of HER2 status 
      in most breast cancer cases.
FAU - Theodosiou, Zenonas
AU  - Theodosiou Z
AD  - Department of Informatics, Aristotle University of Thessaloniki, Box 451, 54124 
      Thessaloniki, Greece.
FAU - Kasampalidis, Ioannis N
AU  - Kasampalidis IN
FAU - Karayannopoulou, Georgia
AU  - Karayannopoulou G
FAU - Kostopoulos, Ioannis
AU  - Kostopoulos I
FAU - Bobos, Mattheos
AU  - Bobos M
FAU - Bevilacqua, Generoso
AU  - Bevilacqua G
FAU - Aretini, Paolo
AU  - Aretini P
FAU - Starita, Antonina
AU  - Starita A
FAU - Lyroudia, Kleoniki
AU  - Lyroudia K
FAU - Pitas, Ioannis
AU  - Pitas I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070921
PL  - Netherlands
TA  - Breast
JT  - Breast (Edinburgh, Scotland)
JID - 9213011
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Monoclonal, Humanized)
RN  - 0 (Antineoplastic Agents)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - P188ANX8CK (Trastuzumab)
SB  - IM
MH  - Antibodies, Monoclonal/administration & dosage
MH  - Antibodies, Monoclonal, Humanized
MH  - Antineoplastic Agents/administration & dosage
MH  - Breast Neoplasms/drug therapy/*metabolism/*pathology
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Italy
MH  - Receptor, ErbB-2/*metabolism
MH  - Signal Processing, Computer-Assisted
MH  - Tissue Array Analysis
MH  - Trastuzumab
EDAT- 2007/09/25 09:00
MHDA- 2008/05/03 09:00
CRDT- 2007/09/25 09:00
PHST- 2006/12/28 00:00 [received]
PHST- 2007/05/30 00:00 [revised]
PHST- 2007/07/12 00:00 [accepted]
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2008/05/03 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
AID - S0960-9776(07)00157-9 [pii]
AID - 10.1016/j.breast.2007.07.041 [doi]
PST - ppublish
SO  - Breast. 2008 Feb;17(1):80-4. doi: 10.1016/j.breast.2007.07.041. Epub 2007 Sep 21.