PMID- 17890487
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20131121
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 15
IP  - 9
DP  - 2007 Sep
TI  - High dietary sodium intake increases white adipose tissue mass and plasma leptin 
      in rats.
PG  - 2200-8
AB  - OBJECTIVE: Salt restriction has been reported to increase white adipose tissue 
      (WAT) mass in rodents. The objective of this study was to investigate the effect 
      of different sodium content diets on the lipogenic and lipolytic activities of 
      WAT. RESEARCH METHODS AND PROCEDURES: Male Wistar rats were fed on normal-sodium 
      (NS; 0.5% Na(+)), high-sodium (HS; 3.12% Na(+)), or low-sodium (LS; 0.06% Na(+)) 
      diets for 3, 6, and 9 weeks after weaning. Blood pressure (BP) was measured using 
      a computerized tail-cuff system. At the end of each period, rats were killed and 
      blood samples were collected for leptin determinations. The WAT from abdominal 
      and inguinal subcutaneous (SC), periepididymal (PE) and retroperitoneal (RP) 
      depots was weighed and processed for adipocyte isolation, rate measurement of 
      lipolysis and d-[U-(14)C]-glucose incorporation into lipids, glucose-6-phosphate 
      dehydrogenase (G6PDH) and malic enzyme activity evaluation, and determination of 
      G6PDH and leptin mRNA expression. RESULTS: After 6 weeks, HS diet significantly 
      increased BP; SC, PE, and RP WAT masses; PE adipocyte size; plasma leptin 
      concentration; G6PDH activity in SC WAT; and PE depots and malic activity only in 
      SC WAT. The leptin levels correlated positively with WAT masses and adipocyte 
      size. An increase in the basal and isoproterenol-stimulated lipolysis and in the 
      ability to incorporate glucose into lipids was observed in isolated adipocytes 
      from HS rats. DISCUSSION: HS diet induced higher adiposity characterized by high 
      plasma leptin concentration and adipocyte hypertrophy, probably due to an 
      increased lipogenic capacity of WAT.
FAU - Fonseca-Alaniz, Miriam H
AU  - Fonseca-Alaniz MH
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, 1524 Prof Lineu Prestes Ave., 05508-900, Sao Paulo, SP, 
      Brazil.
FAU - Brito, Luciana C
AU  - Brito LC
FAU - Borges-Silva, Cristina N
AU  - Borges-Silva CN
FAU - Takada, Julie
AU  - Takada J
FAU - Andreotti, Sandra
AU  - Andreotti S
FAU - Lima, Fabio B
AU  - Lima FB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Leptin)
RN  - 0 (Lipids)
RN  - 0 (Sodium, Dietary)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipocytes/metabolism
MH  - Adipose Tissue, White/*metabolism
MH  - Animal Feed
MH  - Animals
MH  - Blood Pressure
MH  - Body Weight
MH  - Glucose/metabolism
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Leptin/*blood/metabolism
MH  - Lipids/chemistry
MH  - Lipogenesis
MH  - Lipolysis
MH  - Male
MH  - Obesity/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium, Dietary/*pharmacology
EDAT- 2007/09/25 09:00
MHDA- 2008/01/11 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
AID - 15/9/2200 [pii]
AID - 10.1038/oby.2007.261 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2007 Sep;15(9):2200-8. doi: 10.1038/oby.2007.261.