PMID- 17890507
OWN - NLM
STAT- MEDLINE
DCOM- 20080123
LR  - 20091119
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 82
IP  - 6
DP  - 2007 Dec
TI  - Leishmania amazonensis infection impairs differentiation and function of human 
      dendritic cells.
PG  - 1401-6
AB  - Dendritic cells (DCs) are of utmost importance in initiating an immune response 
      and may also function as targets for pathogens. The presence of pathogens inside 
      DCs is likely to impair their functions and thus, influence immune responses. In 
      the present report, we evaluated the impact of the presence of Leishmania 
      amazonensis during differentiation and maturation of human monocyte-derived DCs. 
      The presence of live L. amazonensis parasites during DC differentiation led to a 
      significant decrease in CD80 (92%) and CD1a (56%) expression and an increase in 
      CD86 (56%) cell surface expression. Phenotypic changes were accompanied by a 
      lower secretion of IL-6, observed after 6 days of DC differentiation in the 
      presence of L. amazonensis. DCs differentiated in the presence of L. amazonensis 
      were used as APC in an autologous coculture, and lower amounts of IFN-gamma were 
      obtained compared with control DCs differentiated in the absence of parasites. 
      The effect of heat-killed parasites, but not of Leishmania antigen, during DC 
      differentiation and maturation was similar to that observed with viable 
      parasites. During maturation, the presence of live L. amazonensis parasites, but 
      not of soluble Leishmania antigen, led to a decrease in IL-6 and IL-10 
      production. In this way, we observed that the parasite is able to abrogate full 
      DC differentiation, causing a delay in the immune response and likely, favoring 
      its establishment in human hosts.
FAU - Favali, Cecilia
AU  - Favali C
AD  - Centro de Pesquisas Goncalo Moniz, Fundacao Oswaldo Cruz, Salvador, Bahia, 
      Brazil.
FAU - Tavares, Natalia
AU  - Tavares N
FAU - Clarencio, Jorge
AU  - Clarencio J
FAU - Barral, Aldina
AU  - Barral A
FAU - Barral-Netto, Manoel
AU  - Barral-Netto M
FAU - Brodskyn, Claudia
AU  - Brodskyn C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070921
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Protozoan/immunology
MH  - Cell Adhesion
MH  - *Cell Differentiation
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*cytology/*parasitology
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Leishmania mexicana/cytology/immunology/*physiology
MH  - Monocytes/cytology/parasitology
MH  - Parasites/cytology/immunology/physiology
MH  - Protozoan Infections/*immunology
MH  - Solubility
EDAT- 2007/09/25 09:00
MHDA- 2008/01/24 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2008/01/24 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
AID - jlb.0307187 [pii]
AID - 10.1189/jlb.0307187 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2007 Dec;82(6):1401-6. doi: 10.1189/jlb.0307187. Epub 2007 Sep 21.