PMID- 17891687
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20181201
IS  - 2148-5607 (Electronic)
IS  - 1300-4948 (Linking)
VI  - 18
IP  - 3
DP  - 2007 Sep
TI  - Central effects of glucagon-like peptide-1 on cold-restraint stress-induced 
      gastric mucosal lesions.
PG  - 150-6
AB  - BACKGROUND/AIMS: Intracerebroventricular (i.c.v.) glucagon-like peptide-1 (GLP-1) 
      has been shown to prevent gastric mucosal lesions induced by reserpine and 
      ethanol. Here, we aimed to investigate the effects of i.c.v. GLP-1 on 
      stress-induced gastric mucosal lesions and the mechanisms which may mediate these 
      effects. METHODS: Rats were equipped with intravenous and i.c.v. cannulas under 
      ether anesthesia. To induce cold-restraint stress, rats were kept individually in 
      wire cages, specifically prepared according to their sizes, at 7-9 degrees C for 
      5 hours. They were then decapitated, and their stomachs were removed and scored 
      for mucosal damage. GLP-1 (1, 10, 100, 1000 ng/10 microl; i.c.v.) was injected 5 
      min before cold-restraint stress induction. Rats were pretreated with 
      exendin-(9-39) (2.5 ng/10 microl; i.c.v. and 250 ng/kg; intraperitoneal [i.p.]), 
      calcitonin gene-related peptide (CGRP)-(8-37) (10 microg/kg; i.p.), 
      N(G)-nitro-L-arginine methyl ester (L-NAME) (3 mg/kg; i.v.), indomethacin (5 
      mg/kg; i.p.) and atropine (1 mg/kg; i.p.) to investigate mechanisms which may 
      mediate the gastroprotective effect of GLP-1. RESULTS: GLP-1 (1000 ng/10 microl; 
      i.c.v.) significantly prevented gastric mucosal lesions induced by cold-restraint 
      stress (p<0.01). Intracerebroventricular (i.c.v.), but not i.p., injection of 
      exendin-(9-39) significantly blocked the gastroprotective effect of the peptide 
      (p<0.05). Pretreatment with CGRP-(8-37), L-NAME and indomethacin also prevented 
      the gastroprotective effect of i.c.v. GLP-1 (p<0.05, p<0.05 and p<0.01, 
      respectively), while pretreatment with atropine did not prevent the 
      gastroprotective effect of the peptide. CONCLUSIONS: We conclude that i.c.v GLP-1 
      inhibits the gastric mucosal damage induced by cold-restraint stress via the 
      activation of its specific receptors, and CGRP, nitric oxide and prostaglandins, 
      but not cholinergic muscarinic receptors, mediate this effect.
FAU - Isbil Buyukcoskun, Naciye
AU  - Isbil Buyukcoskun N
AD  - Department of Physiology, Uludag University, School of Medicine, Bursa. 
      nisbil@uludag.edu.tr
FAU - Gulec, Guldal
AU  - Gulec G
FAU - Cam Etoz, Betul
AU  - Cam Etoz B
FAU - Ozluk, Kasim
AU  - Ozluk K
LA  - eng
PT  - Journal Article
PL  - Turkey
TA  - Turk J Gastroenterol
JT  - The Turkish journal of gastroenterology : the official journal of Turkish Society 
      of Gastroenterology
JID - 9515841
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Incretins)
RN  - 0 (Parasympatholytics)
RN  - 0 (Peptide Fragments)
RN  - 119911-68-1 (calcitonin gene-related peptide (8-37))
RN  - 5313W10MYT (exendin (9-39))
RN  - 7C0697DR9I (Atropine)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
MH  - Atropine/pharmacology
MH  - Calcitonin Gene-Related Peptide/pharmacology
MH  - *Cold Temperature
MH  - Enzyme Inhibitors/pharmacology
MH  - Gastric Mucosa/*drug effects/physiopathology
MH  - Glucagon-Like Peptide 1/*pharmacology
MH  - Incretins/*pharmacology
MH  - Indomethacin/pharmacology
MH  - Injections
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Parasympatholytics/pharmacology
MH  - Peptide Fragments/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Restraint, Physical
MH  - Stress, Physiological/*physiology
EDAT- 2007/09/25 09:00
MHDA- 2009/03/04 09:00
CRDT- 2007/09/25 09:00
PHST- 2007/09/25 09:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2007/09/25 09:00 [entrez]
PST - ppublish
SO  - Turk J Gastroenterol. 2007 Sep;18(3):150-6.