PMID- 17892397
OWN - NLM
STAT- MEDLINE
DCOM- 20071211
LR  - 20131121
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 27
IP  - 9
DP  - 2007 Sep
TI  - IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via 
      upregulation of the porcine Arterivirus receptor sialoadhesin.
PG  - 757-66
AB  - The Arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) has a 
      specific tropism for a subset of differentiated macrophages. Porcine sialoadhesin 
      was identified as a PRRSV internalization receptor that is, similarly to 
      sialoadhesins from other species, only expressed on subsets of macrophages. 
      Sialoadhesin is not expressed or only expressed at low levels on monocytes, which 
      might explain why monocytes are largely refractory to PRRSV infection. Different 
      molecules have been identified that regulate human, mouse, or rat sialoadhesin 
      expression in in vitro cultivated monocytes and macrophages, but the effect of 
      these varies greatly between species. In this study, we observed that 
      interferon-alpha (IFN-alpha) induces sialoadhesin expression on monocytes to 
      levels similar as those on macrophages and that it increases sialoadhesin on 
      macrophages. IFN-alpha-induced sialoadhesin expression was shown to be functional 
      using a red blood cell (RBC) binding assay. Furthermore, a 2 or 3 day IFN-alpha 
      pretreatment of monocytes caused a 20-fold increase in the numbers of 
      PRRSV-infected monocytes and increased production of infectious virus. We 
      conclude that IFN-alpha, although it is a potent antiviral molecule, upregulated 
      sialoadhesin infection on in vitro cultivated monocytes, which results in 
      enhanced PRRSV infection of monocytes.
FAU - Delputte, P L
AU  - Delputte PL
AD  - Laboratory of Virology, Department of Virology, Parasitology, and Immunology, 
      Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. 
      peter.delputte@UGent.be
FAU - Van Breedam, W
AU  - Van Breedam W
FAU - Barbe, F
AU  - Barbe F
FAU - Van Reeth, K
AU  - Van Reeth K
FAU - Nauwynck, H J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Cytokines)
RN  - 0 (Interferon-alpha)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Virus)
RN  - 0 (SIGLEC1 protein, human)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - 0 (Siglec1 protein, mouse)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dexamethasone/metabolism
MH  - Interferon-alpha/*pharmacology
MH  - Macrophages, Alveolar/cytology/metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Monocytes/cytology/*metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Virus/*metabolism
MH  - Sialic Acid Binding Ig-like Lectin 1
MH  - Swine
EDAT- 2007/09/26 09:00
MHDA- 2007/12/12 09:00
CRDT- 2007/09/26 09:00
PHST- 2007/09/26 09:00 [pubmed]
PHST- 2007/12/12 09:00 [medline]
PHST- 2007/09/26 09:00 [entrez]
AID - 10.1089/jir.2007.0001 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2007 Sep;27(9):757-66. doi: 10.1089/jir.2007.0001.