PMID- 17892512
OWN - NLM
STAT- MEDLINE
DCOM- 20080115
LR  - 20171116
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 37
IP  - 11
DP  - 2007 Nov
TI  - Differential activation of dendritic cells by nerve growth factor and 
      brain-derived neurotrophic factor.
PG  - 1701-8
AB  - BACKGROUND: Neurotrophins are involved in inflammatory reactions influencing 
      several cells in health and disease including allergy and asthma. Dendritic cells 
      (DCs) play a major role in the induction of inflammatory processes with an 
      increasing role in allergic diseases as well. OBJECTIVE: The aim of this study 
      was to investigate the influence of neurotrophins on DC function. METHODS: 
      Monocyte-derived dendritic cells were generated from allergic and non-allergic 
      donors. Neurotrophin receptors were demonstrated by western blotting, flow 
      cytometry and fluorescence microscopy. Activation of small GTPases was evaluated 
      by pull-down assays. DCs were incubated with nerve growth factor (NGF) and 
      brain-derived neurotrophic factor (BDNF) and supernatants were collected for 
      measurement of IL-4, IL-6, IL-10, IL-12p70, TNF-alpha and TGF-beta. RESULTS: 
      Receptor proteins were detectable by western blot, fluorescence activated cell 
      sorting analysis and fluorescence microscopy. Signalling after neurotrophin 
      stimulation occurred in a ligand-specific pattern. NGF led to decreased RhoA and 
      increased Rac activation, while BDNF affected RhoA and Rac activity in a 
      reciprocal fashion. Cells of allergics released a significantly increased amount 
      of IL-6, while for healthy subjects a significantly higher amount of IL-10 was 
      found. CONCLUSION: These data indicate that DCs are activated by the 
      neurotrophins NGF and BDNF by different pathways in a receptor-dependant manner. 
      These cells then may initiate inflammatory responses based on allergic 
      sensitization releasing preferred cytokines inducing tolerance or a T-helper type 
      2 response.
FAU - Noga, O
AU  - Noga O
AD  - Department of Infectious and Respiratory Diseases; Charite, Universitatsmedizin 
      Berlin, Berlin, Germany. oliver.noga@charite.de
FAU - Peiser, M
AU  - Peiser M
FAU - Altenahr, M
AU  - Altenahr M
FAU - Knieling, H
AU  - Knieling H
FAU - Wanner, R
AU  - Wanner R
FAU - Hanf, G
AU  - Hanf G
FAU - Grosse, R
AU  - Grosse R
FAU - Suttorp, N
AU  - Suttorp N
LA  - eng
PT  - Journal Article
DEP - 20070924
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Antigens, CD)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - CD40 Ligand/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Cytokines/pharmacology
MH  - Dendritic Cells/cytology/*drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Hypersensitivity/blood
MH  - Interleukin-10/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/cytology/drug effects/metabolism
MH  - Nerve Growth Factor/*pharmacology
MH  - Poly I-C/pharmacology
MH  - Receptor, trkA/genetics/metabolism
MH  - Receptor, trkB/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/cytology/drug effects/metabolism
MH  - rac GTP-Binding Proteins/metabolism
MH  - rho GTP-Binding Proteins/metabolism
EDAT- 2007/09/26 09:00
MHDA- 2008/01/16 09:00
CRDT- 2007/09/26 09:00
PHST- 2007/09/26 09:00 [pubmed]
PHST- 2008/01/16 09:00 [medline]
PHST- 2007/09/26 09:00 [entrez]
AID - CEA2832 [pii]
AID - 10.1111/j.1365-2222.2007.02832.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2007 Nov;37(11):1701-8. doi: 10.1111/j.1365-2222.2007.02832.x. 
      Epub 2007 Sep 24.