PMID- 17893905
OWN - NLM
STAT- MEDLINE
DCOM- 20080109
LR  - 20071108
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 110
IP  - 10
DP  - 2007 Nov 15
TI  - Fluorescence in situ hybridization for detecting genomic alterations of cyclin D1 
      and p16 in oral squamous cell carcinomas.
PG  - 2230-9
AB  - BACKGROUND: Cyclin D1 (CCND1) and p16 alterations have been detected in oral 
      squamous cell carcinomas (SCCs), suggesting that abnormalities of these genes may 
      play an important role in the genesis or progression of oral SCCs and serve as 
      independent prognostic indicators. The detection of CCND1 and p16 aberrations 
      using a simple and sensitive method would be valuable for the development of 
      effective treatment modalities for oral cancer. The objective of the current 
      study was to determine whether CCND1 numerical aberrations and p16 deletions in 
      oral SCCs detected by fluorescence in situ hybridization (FISH) have any impact 
      on clinical outcome. METHODS: Using genomic DNA probes for CCND1 and p16, FISH 
      was performed on specimens that were obtained by fine-needle aspiration (FNA) 
      from 57 primary oral SCCs. RESULTS: The CCND1 numerical aberration was observed 
      in 28 of 57 patients (49%) with oral SCCs and was associated significantly with 
      reduced disease-free survival (P = .0004) and overall survival (P = .0179). 
      Conversely, p16 deletion was detected in 22 of 57 patients (39%). The 
      disease-free and overall survival rates for patients with p16 deletion were lower 
      than those among patients without the p16 deletion, although the difference just 
      failed to reach statistical significance (P = .0516 and P = .1878, respectively). 
      The p16 deletion in the presence of the CCND1 numerical aberration conferred 
      significantly worse disease-free survival (P = .0002) and overall survival (P = 
      .0153). CONCLUSIONS: Although the CCND1 numerical aberration was a good predictor 
      of aggressive tumors, recurrence, and poor prognosis in patients with oral SCCs, 
      the authors were able to identify subgroups of patients that had early disease 
      recurrence and a poor prognosis more efficiently by assessment of p16 deletion in 
      addition to CCND1 genetic status using FISH on FNA biopsy samples compared with 
      the analysis of either alteration alone.
CI  - (c) 2007 American Cancer Society.
FAU - Uzawa, Narikazu
AU  - Uzawa N
AD  - Maxillofacial Surgery, Maxillofacial Reconstruction and Function, Division of 
      Maxillofacial and Neck Reconstruction, Graduate School, Tokyo Medical and Dental 
      University, Tokyo, Japan. n-uzawa.mfs@tmd.ac.jp
FAU - Sonoda, Itaru
AU  - Sonoda I
FAU - Myo, Kunihiro
AU  - Myo K
FAU - Takahashi, Ken-Ichiro
AU  - Takahashi K
FAU - Miyamoto, Ryozo
AU  - Miyamoto R
FAU - Amagasa, Teruo
AU  - Amagasa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cyclin D1/*genetics
MH  - Female
MH  - *Genes, p16
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*genetics/pathology
MH  - Recurrence
EDAT- 2007/09/26 09:00
MHDA- 2008/01/10 09:00
CRDT- 2007/09/26 09:00
PHST- 2007/09/26 09:00 [pubmed]
PHST- 2008/01/10 09:00 [medline]
PHST- 2007/09/26 09:00 [entrez]
AID - 10.1002/cncr.23030 [doi]
PST - ppublish
SO  - Cancer. 2007 Nov 15;110(10):2230-9. doi: 10.1002/cncr.23030.