PMID- 17894789
OWN - NLM
STAT- MEDLINE
DCOM- 20080310
LR  - 20220310
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 48
IP  - 1
DP  - 2008 Jan
TI  - Proteomic analysis of supernatant from pooled buffy-coat platelet concentrates 
      throughout 7-day storage.
PG  - 99-107
AB  - BACKGROUND: The platelet (PLT) storage lesion remains incompletely understood. To 
      gain a greater insight into the PLT storage lesion, a proteomic analysis of 
      supernatant from leukofiltered pooled buffy-coat PLT concentrates (PCs) was 
      undertaken. STUDY DESIGN AND METHODS: PCs were prepared in PLT additive solution 
      and stored according to standard blood bank procedures. Supernatant samples were 
      collected throughout 7 days of storage. Maps of supernatant proteins were 
      generated by two-dimensional (2D) gel electrophoresis and mass spectrometry. 
      Cytokine antibody microarrays and enzyme-linked immunosorbent assay were used to 
      investigate bioactive molecules. RESULTS: The 2D gel maps of PC supernatant 
      proteins displayed many features of plasma protein maps. Several storage-induced 
      protein changes were identified including modifications to major plasma proteins. 
      PLT-derived proteins were also identified, including tremlike transcript 1 and 
      integrin-linked kinase, which may influence PLT-endothelium interactions. 
      Cytokine antibody microarrays revealed a number of bioactive proteins that have 
      not been previously associated with PCs produced for transfusion, such as 
      brain-derived neurotrophic factor (BDNF). The concentration of PLT-derived 
      cytokines including BDNF, CXCL7, epidermal growth factor, PLT-derived growth 
      factor (PDGF), and CCL5 significantly increased during storage of PCs. Extended 
      storage from Day 5 to Day 7 caused significantly increased levels of BDNF, PDGF, 
      and CCL5 in PC supernatant. CONCLUSION: Proteomic techniques provide valuable new 
      insight into the effects of storage on PCs and the contribution of soluble 
      proteins to the development of the PLT storage lesion and recipient responses to 
      PLT transfusion.
FAU - Glenister, Kristen M
AU  - Glenister KM
AD  - Research Unit, Australian Red Cross Blood Service, Melbourne, Victoria, 
      Australia.
FAU - Payne, Katherine A
AU  - Payne KA
FAU - Sparrow, Rosemary L
AU  - Sparrow RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070924
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Proteins)
SB  - IM
MH  - Blood Platelets/*chemistry
MH  - *Blood Preservation
MH  - Culture Media, Conditioned/chemistry
MH  - Cytokines/analysis
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Leukocyte Reduction Procedures
MH  - Mass Spectrometry
MH  - Platelet Transfusion
MH  - Proteins/*analysis
MH  - Proteomics/*methods
EDAT- 2007/09/27 09:00
MHDA- 2008/03/11 09:00
CRDT- 2007/09/27 09:00
PHST- 2007/09/27 09:00 [pubmed]
PHST- 2008/03/11 09:00 [medline]
PHST- 2007/09/27 09:00 [entrez]
AID - TRF01487 [pii]
AID - 10.1111/j.1537-2995.2007.01487.x [doi]
PST - ppublish
SO  - Transfusion. 2008 Jan;48(1):99-107. doi: 10.1111/j.1537-2995.2007.01487.x. Epub 
      2007 Sep 24.