PMID- 17896795 OWN - NLM STAT- MEDLINE DCOM- 20080428 LR - 20231213 IS - 1097-4547 (Electronic) IS - 0360-4012 (Linking) VI - 86 IP - 3 DP - 2008 Feb 15 TI - Characterization and use of the NSC-34 cell line for study of neurotrophin receptor trafficking. PG - 553-65 AB - This study addressed the suitability of the NSC-34 cell line as a motor neuron-like model for investigating neurotrophin receptor trafficking and associated subcellular processes. Initially, culture conditions were optimized for the use of NSC-34 cells in confocal microscopy. Cell surface markers, as well as markers associated with the regulated endosomal pathway thought to be associated with neurotrophin receptor transport, were identified. The study revealed the presence of a number of molecules previously not described in the literature, including the tropomyosin-like receptor kinase C (TrkC), sortilin, the vesicular acetylcholine transporter (VAChT), and the lipid raft-associated ganglioside GT1b. The presence of both sortilin and Gt1b was of special interest, insofar as these markers have been implicated in direct relationships with the p75NTR receptor. Evidence is provided for neurotrophin-dependent internalization of p75NTR and TrkB. Both nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) increased the rate of internalization of p75NTR, with internalization dynamics comparable to those described for other cell lines. Thus, these studies not only describe components of the regulatory process governing the trafficking of this important receptor but also clearly demonstrate the value of NSC-34 cells as a suitable motor neuron model for the study of internalization and trafficking of cell surface molecules. CI - (c) 2007 Wiley-Liss, Inc. FAU - Matusica, Dusan AU - Matusica D AD - Centre for Neuroscience, Department of Human Physiology, Flinders University, Bedford Park, South Australia, Australia. dusan.matusica@flinders.edu.au FAU - Fenech, Matthew P AU - Fenech MP FAU - Rogers, Mary-Louise AU - Rogers ML FAU - Rush, Robert A AU - Rush RA LA - eng PT - Journal Article PL - United States TA - J Neurosci Res JT - Journal of neuroscience research JID - 7600111 RN - 0 (Antibodies) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Culture Media) RN - 0 (Gangliosides) RN - 0 (Membrane Proteins) RN - 0 (Receptors, Nerve Growth Factor) RN - 0 (Ngfr protein, mouse) RN - 59247-13-1 (trisialoganglioside GT1) RN - 9061-61-4 (Nerve Growth Factor) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Antibodies/immunology MH - Brain-Derived Neurotrophic Factor/pharmacology MH - Cell Differentiation MH - *Cell Line/cytology/drug effects MH - Culture Media/pharmacology MH - Cytosol/metabolism MH - Endocytosis/drug effects MH - Gangliosides/immunology MH - Membrane Proteins/metabolism MH - Models, Neurological MH - Motor Neurons/metabolism MH - Nerve Growth Factor/pharmacology MH - Protein Transport MH - Receptor, trkB/immunology/metabolism MH - Receptors, Nerve Growth Factor/drug effects/immunology/*metabolism EDAT- 2007/09/28 09:00 MHDA- 2008/04/29 09:00 CRDT- 2007/09/28 09:00 PHST- 2007/09/28 09:00 [pubmed] PHST- 2008/04/29 09:00 [medline] PHST- 2007/09/28 09:00 [entrez] AID - 10.1002/jnr.21507 [doi] PST - ppublish SO - J Neurosci Res. 2008 Feb 15;86(3):553-65. doi: 10.1002/jnr.21507.