PMID- 17897187
OWN - NLM
STAT- MEDLINE
DCOM- 20080414
LR  - 20070927
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 103
IP  - 4
DP  - 2007 Oct
TI  - Quantification of Leuconostoc populations in mixed dairy starter cultures using 
      fluorescence in situ hybridization.
PG  - 855-63
AB  - AIMS: Development of a rapid method to identify and quantify Leuconostoc 
      populations in mesophilic starter cultures. METHODS AND RESULTS: 16S 
      rRNA-targeted oligonucleotide probes were used in a whole cell in situ 
      hybridization assay for the identification of the genus Leuconostoc and an 
      undescribed Leuconostoc ribospecies. The probes were fluorescently labelled and 
      used to quantify the Leuconostoc populations in five different mixed starter 
      cultures. CONCLUSIONS: There was a good correlation between the results obtained 
      using fluorescence in situ hybridization (FISH) with that of standard plate 
      counting methods. SIGNIFICANCE AND IMPACT OF THE STUDY: To develop a FISH method 
      capable of identifying and quantifying the Leuconostoc population in starter 
      cultures within 1 day.
FAU - Olsen, K N
AU  - Olsen KN
AD  - Chr. Hansen A/S, Boge Alle 10-12, Horsholm, Denmark. kna@kvl.dk
FAU - Brockmann, E
AU  - Brockmann E
FAU - Molin, S
AU  - Molin S
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Cheese/*microbiology
MH  - Fermentation
MH  - *Food Microbiology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Leuconostoc/classification/genetics/growth & development/*isolation & 
      purification
MH  - Oligonucleotide Probes
MH  - Permeability
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
EDAT- 2007/09/28 09:00
MHDA- 2008/04/15 09:00
CRDT- 2007/09/28 09:00
PHST- 2007/09/28 09:00 [pubmed]
PHST- 2008/04/15 09:00 [medline]
PHST- 2007/09/28 09:00 [entrez]
AID - JAM3298 [pii]
AID - 10.1111/j.1365-2672.2007.03298.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2007 Oct;103(4):855-63. doi: 10.1111/j.1365-2672.2007.03298.x.