PMID- 17897558
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20070927
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 43
IP  - 7
DP  - 2007 Jul
TI  - [The structure and viability analysis of corneal epithelial flap in the rabbit 
      cornea after Epi-LASIK].
PG  - 651-7
AB  - OBJECTIVE: To evaluate the changes of ultrastructure and viability in the rabbit 
      corneal epithelial flap after Epi-LASIK (epiploic laser in-situ keratomileusis) 
      surgery and its effect on keratocyte apoptosis and proliferation of peripheral 
      corneal epithelium (out of corneal epithelial flap). METHODS: Fifty-eighty eyes 
      of 29 New Zealand rabbits were used, Epi-LASIK was performed in 28 eyes and 
      Photorefractive keratectomy (PRK) was carried out in 24 eyes. treated-eyes were 
      randomly divided into four groups and were sacrificed at 1, 3, 5, 7 days after 
      surgery, six eyes without treatment were served as blank controls. Histological 
      structure from The specimens of Epi-LASIK and controls eyes were assessed by 
      light, transmission electron microscopy; epithelial cells viability were assessed 
      by enzyme-histochemistry and Immunohistochemistry staining (proliferating cell 
      nuclear antigen, PCNA) were performed to detect proliferation of peripheral 
      corneal epithelial cells. Apoptotic cells were detected by TUNEL assay 
      (TdT-mediated dUTP nick-end labeling) from the specimens of Epi-LASIK and PRK. 
      RESULTS: The study from Transmission electron microscopy demonstrated that 
      epithelial flap separated by KM5000D type epikeratome retained its typical 
      stratification and integrity and the basement membrane including lamina densa and 
      lamina lucida were compaginated with stroma. The expression of ATP enzyme, G-6-P 
      enzyme from epithelial flap to peripheral epithelium of Epi-LASIK-treated eyes 
      were (79%, 58%, 69%, 86%), (79%, 63%, 77%, 97%) at 1, 3, 5, 7 days after surgery 
      respectively. There was no statistically significant difference in the cell 
      Viability and the number of PCNA in peripheral epithelial cells among four 
      Epi-LASIK groups and control group. At 1 day after surgery, no difference in 
      TUNEL positive cells were seen between specimens of Epi-LASIK (3.429 +/- 1.693) 
      and PRK (3.796 +/- 1.998); At 3, 5, 7 days, there was a significant difference in 
      the number of keratocyte apoptosis in PRK compared to Epi-LASIK specimens. 
      CONCLUSIONS: Epithelial flap separated by KM5000D type epikeratome retained its 
      typical stratification and integrity. The flap keeps high viability and no 
      peripheral epithelial cell proliferation and therefore it may play a role in the 
      inhibition of keratocyte apoptosis.
FAU - Liu, Wei-feng
AU  - Liu WF
AD  - Ophthalmic Center, the Second Affiliated Hospital, Chongqing University of 
      Medical Sciences, Chongqing 400010, China.
FAU - DU, Zhi-yu
AU  - DU ZY
FAU - Zhao, Wu-xiao
AU  - Zhao WX
FAU - Huang, Zheng
AU  - Huang Z
FAU - Liu, De-jie
AU  - Liu DJ
FAU - Chen, Ying
AU  - Chen Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cornea/surgery
MH  - Epithelium, Corneal/*metabolism/*pathology/transplantation/ultrastructure
MH  - Female
MH  - Hyperplasia
MH  - *Keratomileusis, Laser In Situ
MH  - Male
MH  - Rabbits
MH  - Surgical Flaps/*pathology
EDAT- 2007/09/28 09:00
MHDA- 2010/11/05 06:00
CRDT- 2007/09/28 09:00
PHST- 2007/09/28 09:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
PHST- 2007/09/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2007 Jul;43(7):651-7.